Cells were imaged at room temperature using a Nikon Ti-E inverted microscope (Nikon Instruments) outfitted with a TIRF 60X/1.49 NA oil objective, an Andor Laser Combiner and an electron-multiplying charge-coupled device camera (iXon ULTRA 897BV; Andor Technology). Samples were excited with a 488nm laser line, and emission was collected through a single band-pass filter centered on 510 nm. All images were acquired using iQ3 acquisition software (Andor Technology). The depth of the evanescent field was approximately 150 nm.
Ixon ultra 897bv
Manufactured by Oxford Instruments
The IXon ULTRA 897BV is a high-performance, back-illuminated, electron-multiplying CCD (EMCCD) camera designed for low-light imaging applications. It features a 1024 x 1024 pixel sensor with a pixel size of 13 μm and a quantum efficiency of up to 95%. The camera provides high-speed readout rates up to 17 MHz and advanced on-chip electron multiplication to enhance signal-to-noise ratio in low-light conditions.
Automatically generated - may contain errors
2 protocols using ixon ultra 897bv
1
TIRF Microscopy of Cell Samples
2
TIRF Microscopy of Cell Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were imaged at room temperature using a Nikon Ti-E inverted microscope (Nikon Instruments) outfitted with a TIRF 60X/1.49 NA oil objective, an Andor Laser Combiner and an electron-multiplying charge-coupled device camera (iXon ULTRA 897BV; Andor Technology). Samples were excited with a 488nm laser line, and emission was collected through a single band-pass filter centered on 510 nm. All images were acquired using iQ3 acquisition software (Andor Technology). The depth of the evanescent field was approximately 150 nm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!