Total pdgfr β

Unless otherwise specified, all materials were reagent grade and obtained from Sigma-Aldrich (St. Louis, MO, USA). Anti-Ki67 antibody was obtained from BD Biosciences (San Jose, CA, USA). Alkaline phosphatase (ALP) conjugated horse anti-mouse IgG antibody was obtained from Vector Laboratory (Burlingame, CA, USA). Anti–phosphorylated/total AMPKα, anti-phosphorylated/total acetyl-CoA carboxylase (ACC) and anti-phosphorylated PDGF receptor β (PDGFRβ) antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). Anti-phosphorylated/total CREB1, c-Src and total PDGFR-β antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-phosphorylated tyrosine antibody was obtained from Millipore (Billerica, MA, USA).

C-ANP _4–23 was purchased from Bachem, antibodies against Cyclin D1, Cdk4, total EGF-R, phospho-EGFR, total PDGF-R β, phospho-PDGF-R β, total IGF-R β, phospho-IGF-IR, c-Src, phosphor-c-Src, horseradish peroxidase-conjugated goat anti-mouse/anti-rabbit and anti-goat immunoglobulin were from Santa Cruz Biotechnology Inc (Santa Cruz, CA, USA), antibodies against Nox1, Nox2, Nox4 and p47^phox were from EMD Millipore. Thymidine, [Methyl-3H] was from PerkinElmer, Inc. (Massachusetts, U.S.A.). All other chemicals were purchased from Sigma Aldrich Canada.