Dpmanager software v2

SCID mice (Charles River Laboratories, L’Arbresle, France) were inoculated subcutaneously with 1.2×10⁷ cells of RPMI-8226 cell line. Mice were randomly assigned into cohorts of 5 mice each and received either a twice-daily dose of CPI203 (2.5 mg/kg) for two weeks, or a daily dose of lenalidomide (25 mg/kg) plus twice weekly dexamethasone (1 mg/kg), or the combination of both, or an equal volume of vehicle. Twenty-one days post-cell inoculation, intratumoral glucose uptake was evaluated with an Odyssey infra-red scanner (Li-Cor, Lincoln, NE, USA) in mice previously injected with an IRDye 800CW 2-deoxyglucose probe (Li-Cor). Animals were then sacrificed according to institutional guidelines and tumor xenografts were isolated. Paraffin-embedded tumor samples were subjected to immunohistochemical staining using primary antibodies against Ikaros, MYC, IRF4, GADD45B and pH3 and evaluated with an Olympus DP70 microscope by means of a 20×/0.75 NA objective and DPManager software v.2.1.1 (Olympus).