OVA-specific IgG1 antibodies in mouse serum were determined by ELISA. 96-well plates were coated with 100 µg/ml OVA overnight at 4 °C. Serum samples were titrated onto the plates and detected with goat anti-mouse IgG1 antiserum (Southern Biotech, Birmingham, USA) coupled to biotin. The ELISA was developed with avidin-HRP/TMB and values measured at 450 nm after addition of 0.75 µl H2SO4. All values are expressed relative to a standard from pooled sera of C57BL/6 mice immunized and boosted with OVA/IFA.
Goat anti mouse igg1 antiserum
Manufactured by Southern Biotech
Sourced in United States
Goat anti-mouse IgG1 antiserum is a laboratory reagent used to detect and quantify the presence of mouse IgG1 antibodies in various experimental and analytical procedures.
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2 protocols using goat anti mouse igg1 antiserum
1
OVA-Specific IgG1 Antibody Quantification
2
Quantification of OVA-specific IgG1 in Mice
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OVA-specific IgG1 antibodies in mouse serum were determined by ELISA. 96-well plates were coated with 100 μg/ml overnight at 4°C. Serum samples were titrated onto the plates and detected with goat anti-mouse IgG1 antiserum (Southern Biotech, Birmingham, USA) coupled to biotin. The ELISA was developed with avidin-HRP/TMB and values measured at 450 nm after the addition of 0.75 μl of H2SO4. All values are expressed related to a standard from pooled sera of mice immunized and boosted with OVA/IFA.
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