U251-MG cells were grown on glass coverslips until they were 50~70% confluent. After 24 hrs, the cells were fixed in 4% paraformaldehyde at room temperature for 10 min and permeabilized in 0.2% Triton X100 for 5 min at room temperature. Then cells were incubated in blocking buffer containing 5% bovine serum albumin (Sigma-Aldrich) in 1 X TBS for 1 hr at 37°C. The rabbit polyclonal anti-TMEM39A was diluted 200-fold for primary antibody and incubated for overnight. The secondary antibody, FITC-conjugated anti-rabbit antibody (BD Biosciences, NJ, USA) was used. After appropriate rinsing, cover slips were mounted with Vectashield (Vector Laboratories, CA, USA) and visualized using a Zeiss confocal microscope.
Fitc conjugated anti rabbit antibody
Manufactured by BD
Sourced in United States
The FITC-conjugated anti-rabbit antibody is a laboratory reagent used for the detection and identification of rabbit-derived proteins or antigens in various research and diagnostic applications. The antibody is labeled with the fluorescent dye FITC (Fluorescein Isothiocyanate), which allows for the visualization and localization of the target analyte when viewed under a fluorescence microscope or detected using a fluorescence-based assay.
Automatically generated - may contain errors
3 protocols using fitc conjugated anti rabbit antibody
1
Immunofluorescence Imaging of TMEM39A
2
Immunocytochemical Localization of TrioBP
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U251-MG cells were grown on glass coverslips until they were 50–70% confluent. After 24 h, the cells were fixed in 4% paraformaldehyde at room temperature for 10 min and permeabilized in 0.2% Triton X-100 for 5 min at room temperature. Then cells were incubated in blocking buffer containing 5% bovine serum albumin (Sigma-Aldrich; Merck KGaA) in 1X TBS for 1 h at 37°C. The rabbit polyclonal anti-TrioBP was diluted 200-fold for primary antibody and incubated for overnight. The secondary antibody, FITC-conjugated anti-rabbit antibody (BD Biosciences, Franklin Lakes, NJ, USA) was used. After appropriate rinsing, cover slips were mounted with Vectashield (Vector Laboratories, Inc., Burlingame, CA, USA) and visualized using a Zeiss confocal microscope (Zeiss AG, Oberkochen, Germany).
3
Immunofluorescent Localization of AMACR
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U343-MG cells were grown on glass coverslips until they were 50–70% confluent. After 24 h, the cells were fixed in 4% (w/v) paraformaldehyde at room temperature for 10 min and permeabilized in 0.2% (v/v) Triton X100 for 5 min at room temperature. Then cells were incubated in blocking buffer containing 5% (v/v) bovine serum albumin (Sigma) in 1 X TBS for 1 h at 37°C. The rabbit polyclonal anti-AMACR was diluted 200-fold for primary antibody and incubated for overnight. The secondary antibody, FITC-conjugated anti-rabbit antibody (BD Biosciences) was used. After appropriate rinsing, cover slips were mounted with Vectashield (Vector Laboratories) and visualized using a Leica confocal microscope.
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