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Ovcar5

Manufactured by Corning
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OVCAR5 is a laboratory instrument designed for scientific research and experimentation. It is a type of cell culture incubator used to maintain and cultivate cell lines. The OVCAR5 provides a controlled environment for cell growth, including temperature, humidity, and gas composition regulation. This product is intended for use in research settings by trained professionals.

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Lab products found in correlation

Medium 199, by Thermo Fisher Scientific (3 mentions) A2780, by European Collection of Cell Cultures (3 mentions) Bright line hemacytometer, by Merck Group (3 mentions) A2780, by Corning (3 mentions) Ovcar5, by American Type Culture Collection (3 mentions) Mycoalert mycoplasma detection kit, by Lonza (3 mentions) Caov3, by American Type Culture Collection (2 mentions) Rpmi 1640, by Corning (2 mentions) Caov3, by Corning (2 mentions) Fetal bovine serum (fbs), by Gemini Bio (1 mentions) Penicillin streptomycin solution, by Corning (1 mentions) Rpmi 1640 medium, by Corning (1 mentions) Mcdb 105, by Cell Applications (1 mentions)

3 protocols using ovcar5

1

Cultivation of Ovarian Cancer Cell Lines

Cited 1 time
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Human ovarian cancer cell line OVCAR5 (female) was obtained from American Type Culture Collection. A2780 (female) was acquired from European Collection of Cell Cultures. OVCA433 (female) ovarian serous adenocarcinoma cell line was a generous gift from Dr. Marcin Iwanicki (Stevens Institute of Technology, Department of Chemistry and Chemical Biology, NJ USA). OVCAR5 and A2780 were grown in RPMI-1640 medium (Corning, catalogue No. 10-040-CM). OVCA433 (Iwanicki et al., 2016)39 (link) were cultured in 1:1 mixture of MCDB 105 (Cell Applications, catalogue No. 117–500) medium and Medium 199 (Gibco, catalogue No. 11150059). All media were supplemented with fetal bovine serum (FBS; Gemini Bio-products, catalogue No. 900–208) and penicillin-streptomycin solution (Corning, catalogue No. 30-002-Cl) at the final concentrations of 10% and 1%, respectively, unless stated otherwise. Cells were kept in a humidified incubator with 5% CO2 at 37 °C. All cells were tested for mycoplasma infection (MycoAlert Mycoplasma Detection Kit, Lonza, catalogue No. LT07-218) and found to be negative. Cells were counted by means of Bright-Line™ Hemacytometer (Sigma, catalogue No. Z359629).
Zaborowski M.P., Cheah P.S., Zhang X., Bushko I., Lee K., Sammarco A., Zappulli V., Maas S.L., Allen R.M., Rumde P., György B., Aufiero M., Schweiger M.W., Lai C.P., Weissleder R., Lee H., Vickers K.C., Tannous B.A, & Breakefield X.O. (2019). Membrane-bound Gaussia luciferase as a tool to track shedding of membrane proteins from the surface of extracellular vesicles. Scientific Reports, 9, 17387.
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2

Ovarian Cancer Cell Line Cultivation

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Human ovarian cancer cell lines, CaOV3 (female), OV90 (female) and OVCAR5 (female) were obtained from American Type Culture Collection. A2780 (female) was acquired from European Collection of Cell Cultures. Kuramochi (female) and OVSAHO (female) cells were a generous gift from Dr. Kristi Egland (Sanford Research, South Dakota). Primary high grade ovarian cancer cells (DF30, female) were isolated as previously described (Davidowitz et al., 2014 (link)). CaOV3 were cultured in Dulbecco’s modified essential medium (Corning, catalog No. 10–013-CV). OV90, OVCAR5 and A2780 were grown in RPMI-1640 medium (Corning, catalog No. 10–040-CM). KURAMOCHI, OVSAHO, DF30 (Iwanicki et al., 2016 ) were cultured in 1:1 mixture of MCDB 105 (Cell Applications, catalog No. 117–500) medium and Medium 199 (GIBCO, catalog No. 11150059). All media were supplemented with fetal bovine serum (Gemini Bio-products, catalog No. 900–208) and penicillin-streptomycin solution (Corning, catalog No. 30–002-Cl) at the final concentrations 10% and 1%, respectively. Cells were kept in a humidified incubator with 5% CO2 at 37°C. All cells were tested for mycoplasma infection (MycoAlert Mycoplasma Detection Kit, Lonza, catalog No. LT07–218) and found to be negative. Cells were counted by means of Bright-Line Hemacytometer (Sigma, catalog No. Z359629).
Zaborowski M.P., Lee K., Na Y.J., Sammarco A., Zhang X., Iwanicki M., Cheah P.S., Lin H.Y., Zinter M., Chou C.Y., Fulci G., Tannous B.A., Lai C.P., Birrer M.J., Weissleder R., Lee H, & Breakefield X.O. (2019). Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles. Cell reports, 27(1), 255-268.e6.
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3

Ovarian Cancer Cell Line Cultivation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human ovarian cancer cell lines, CaOV3 (female), OV90 (female) and OVCAR5 (female) were obtained from American Type Culture Collection. A2780 (female) was acquired from European Collection of Cell Cultures. Kuramochi (female) and OVSAHO (female) cells were a generous gift from Dr. Kristi Egland (Sanford Research, South Dakota). Primary high grade ovarian cancer cells (DF30, female) were isolated as previously described (Davidowitz et al., 2014 (link)). CaOV3 were cultured in Dulbecco’s modified essential medium (Corning, catalog No. 10–013-CV). OV90, OVCAR5 and A2780 were grown in RPMI-1640 medium (Corning, catalog No. 10–040-CM). KURAMOCHI, OVSAHO, DF30 (Iwanicki et al., 2016 ) were cultured in 1:1 mixture of MCDB 105 (Cell Applications, catalog No. 117–500) medium and Medium 199 (GIBCO, catalog No. 11150059). All media were supplemented with fetal bovine serum (Gemini Bio-products, catalog No. 900–208) and penicillin-streptomycin solution (Corning, catalog No. 30–002-Cl) at the final concentrations 10% and 1%, respectively. Cells were kept in a humidified incubator with 5% CO2 at 37°C. All cells were tested for mycoplasma infection (MycoAlert Mycoplasma Detection Kit, Lonza, catalog No. LT07–218) and found to be negative. Cells were counted by means of Bright-Line Hemacytometer (Sigma, catalog No. Z359629).
Zaborowski M.P., Lee K., Na Y.J., Sammarco A., Zhang X., Iwanicki M., Cheah P.S., Lin H.Y., Zinter M., Chou C.Y., Fulci G., Tannous B.A., Lai C.P., Birrer M.J., Weissleder R., Lee H, & Breakefield X.O. (2019). Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles. Cell reports, 27(1), 255-268.e6.
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