Macs tubes

Manufactured by Miltenyi Biotec

MACS tubes are specialized laboratory tubes designed for use with Miltenyi Biotec's magnetic cell separation systems. They provide a containment solution for the cell suspension during the separation process.

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MACS C tube (32 citations) MACS M Tubes (5 citations) Gentle MACS tubes C (5 citations)

2 protocols using macs tubes

Lung Lymphocyte Isolation by Percoll Gradient

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Lungs were harvested and tissue was dissociated in Miltenyi MACS tubes with collagenase (1 mg/mL) and DNase (1 μg/mL) for 25 min at 37°C. Digested lungs were resuspended in 5 mL of 40% Percoll in RPMI 1640, and 3 mL of 66% Percoll in phosphate-buffered saline (PBS) was then underlaid (catalog number 17-0891-01; GE Healthcare). Samples were spun for 20 min at 2,000 rpm at room temperature. Lymphocytes were then harvested from the buffy coat layer and resuspended in complete RPMI 1640.

Kohn E.M., Taira C., Dobson H., Dias L.D., Okaa U., Wiesner D.L., Wüthrich M, & Klein B.S. (2022). Variation in Host Resistance to Blastomyces dermatitidis: Potential Use of Genetic Reference Panels and Advances in Immunophenotyping of Diverse Mouse Strains. mBio, 13(1), e03400-21.

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Immune Cell Isolation from Murine Tissues

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Spleens and brachial lymph nodes were harvested either 7, 8, 14, or 28
days post vaccination, or 4 days post infection. Spleen and draining lymph nodes
were then mashed through 40 μm filters and washed with 5 ml cold wash
buffer (RPMI with 1% FBS, 1% penicillin and streptomycin). Splenocytes were
subjected to red blood cell lysis with 3 ml ACK lysis buffer for 3 minutes at
room temperature, stopped with 5 ml complete RPMI (RPMI with 10% FBS, 1%
penicillin and streptomycin). Lungs were harvested after challenge and
dissociated in Miltenyi MACS tubes and digested with collagenase (1 mg/ml) and
DNase (1 μg/ml) for 25 minutes at 37°C. Digested lungs were
resuspended in 5 ml of 40% Percoll in RPMI; 3 ml of 66% percoll in PBS was
underlaid (GE healthcare 17–0891-01). Samples were spun for 20 min at
2000 rpm at room temperature. Lymphocytes were then harvested from the buffy
coat layer and resuspended in complete RPMI.

Kohn E.M., dos Santos Dias L., Dobson H.E., He X., Wang H., Klein B.S, & Wüthrich M. (2022). SLAMF1 is dispensable for vaccine induced T cell development but required for resistance to fungal infection. Journal of immunology (Baltimore, Md. : 1950), 208(6), 1417-1423.

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