The wild-type (WT) and mutant (MUT) luciferase reporter vectors of the EIF4G2 3′-UTR were obtained from GeneChem. A total of 1 × 104 293 T cells were seeded into 96-well plates, and then the WT and MUT reporter vectors and miR-144 overexpression vector were cotransfected with Lipofectamine 3000 into 293 T cells. Forty-eight hours later, the firefly and Renilla luciferase activities were assessed by a GloMax microplate luminometer (Promega, USA) using a Dual-Glo® Luciferase Assay System kit (E2920, Promega, USA). Renilla luciferase activities were used to evaluate transfection efficiency.
Eif4g2 3 utr
Manufactured by Genechem
EIF4G2 3'-UTR is a genetic sequence that represents the 3' untranslated region of the eIF4G2 gene. This sequence can be used as a research tool in molecular biology and genetics studies.
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4 protocols using eif4g2 3 utr
1
Luciferase reporter assay for EIF4G2 3'-UTR
2
Luciferase reporter assay for EIF4G2 3'-UTR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The wild-type (WT) and mutant (MUT) luciferase reporter vectors of EIF4G2 3'-UTR were obtained from GeneChem. 1x10 4 293T cells were seeded into 96-well plates, then the WT and MUT reporter vectors and miR-144 overexpression vector were co-transfected with Lipofectamine 3000 into 293T cells. 48 h later, the firefly and Renilla luciferase activities were assessed by GloMax microplate luminometer (Promega, USA) using Dual-Glo® Luciferase Assay System kit (E2920, Promega, USA). Renilla luciferase activities were used to evaluate transfection efficiency
3
Luciferase Assay of EIF4G2 3'-UTR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The wild-type (WT) and mutant (MUT) luciferase reporter vectors of the EIF4G2 3'-UTR were obtained from GeneChem. A total of 1x10 4 293T cells were seeded into 96-well plates, and then the WT and MUT reporter vectors and miR-144 overexpression vector were cotransfected with Lipofectamine 3000 into 293T cells. Forty-eight hours later, the re y and Renilla luciferase activities were assessed by a GloMax microplate luminometer (Promega, USA) using a Dual-Glo® Luciferase Assay System kit (E2920, Promega, USA). Renilla luciferase activities were used to evaluate transfection e ciency.
4
Luciferase Assay of EIF4G2 3'-UTR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The wild-type (WT) and mutant (MUT) luciferase reporter vectors of the EIF4G2 3'-UTR were obtained from GeneChem. A total of 1x10 4 293T cells were seeded into 96-well plates, and then the WT and MUT reporter vectors and miR-144 overexpression vector were cotransfected with Lipofectamine 3000 into 293T cells. Forty-eight hours later, the re y and Renilla luciferase activities were assessed by a GloMax microplate luminometer (Promega, USA) using a Dual-Glo® Luciferase Assay System kit (E2920, Promega, USA). Renilla luciferase activities were used to evaluate transfection e ciency.
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