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Monoclonal rabbit anti parp1

Manufactured by Cell Signaling Technology
Sourced in United States

Monoclonal rabbit anti-PARP1 is a laboratory reagent that can be used to detect the presence and levels of PARP1 protein in biological samples. PARP1 is an enzyme involved in various cellular processes.

Automatically generated - may contain errors

2 protocols using monoclonal rabbit anti parp1

1

Antibody Immunoprecipitation Protocol

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Antibodies to polyclone rabbit anticaspase3, monoclonal rabbit anti-PARP1, and mouse anti-Flag were obtained from Cell Signaling Technology (USA); polyclonal mouse anti-Cul4a, monoclonal rabbit anti-α-tubulin, and anti-GAPDH were obtained from Proteintech (Wuhan, China). Protein A/G magnetic beads were acquired from Biotool (Shanghai, China).
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2

Antibody Immunoblotting Assay Protocol

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Antibodies included polyclonal goat anti-Septin4 (1:1000; abcam, USA), polyclonal rabbit anti-WWP2 (1:1000; abcam; 1:500; Proteintech, USA), monoclonal rabbit anti-HA (1:1000; Cell Signaling Technology, USA), monoclonal rabbit anti-Myc (1:1000; Cell Signaling Technology), monoclonal rabbit anti-Flag (1:1000; abcam), polyclonal rabbit anti-caspase-3 (1:1000; Cell Signaling Technology), monoclonal rabbit anti-PARP1 (1:1000; Cell Signaling Technology), polyclonal rabbit anti-collagen I (1:1000; Proteintech), polyclonal rabbit anti-aerfa5-SMA (1:1000; Proteintech), polyclonal rabbit anti-OGG1 (1:500; Proteintech), polyclonal rabbit anti-SOD1 (1:500; Proteintech), monoclonal mouse anti-3-nitrotyrosine (1:1000; abcam), monoclonal mouse anti-β-tubulin (1:2000; abcam), and monoclonal mouse anti-GAPDH (1:2000; abcam). MG132 was reconstituted in DMSO (10 μM), which was obtained from Biotool (USA). CHX was obtained from Biotool and used at 10 μM in DMSO.
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