Plasma cytokine levels at midgestation were measured using the Bio-Plex Pro™ Mouse Cytokine Th17 Panel A 6-Plex kit (Bio-Rad; Hercules, CA), read using the Bio-Rad Bio-Plex® 200 system (Bio-Rad; Hercules, CA), and analysed with Bio-Plex Manager™ software (Bio-Rad; Hercules, CA).
Bio plex pro mouse cytokine th17 panel a 6 plex kit
Manufactured by Bio-Rad
Sourced in Germany, United States
The Bio-Plex Pro™ Mouse Cytokine Th17 Panel A 6-Plex kit is a multiplex assay designed to quantify the levels of six mouse cytokines simultaneously in a single sample. The kit utilizes bead-based technology to measure the concentrations of interleukin-17A (IL-17A), interleukin-17F (IL-17F), interleukin-21 (IL-21), interleukin-22 (IL-22), interleukin-23 (IL-23), and interleukin-6 (IL-6).
Automatically generated - may contain errors
Lab products found in correlation
Bio plex manager software, by Bio-Rad (2 mentions)
Il 6 and il 1β, by Thermo Fisher Scientific (2 mentions)
Protein assay reagent, by Bio-Rad (2 mentions)
Tnf α, by BD (2 mentions)
Bio plex 200 system, by Bio-Rad (1 mentions)
Liquichip 200 workstation, by Qiagen (1 mentions)
Serum gel z tubes, by Sarstedt (1 mentions)
Bio plex 200 luminex machine, by Bio-Rad (1 mentions)
Hmgb1, by Fujifilm (1 mentions)
Hmgb1, by Shino-Test (1 mentions)
5 protocols using bio plex pro mouse cytokine th17 panel a 6 plex kit
1
Midgestation Plasma Cytokine Profiling
2
Cytokine Profiling in Cardiomyocyte and Tissue
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Cardiomyocyte supernatant was analyzed by ELISA kits for interleukin (IL)-6 and tumor necrosis factor-α (TNF-α) (BD Biosciences, San Jose, CA) according to the manufacturer’s instructions. Cardiac and lung tissue was crushed in liquid nitrogen, and equal weights of powdered tissues (~ 50 mg) were dissolved in 500 µl of lysis buffer (10 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM DTT, 1% Triton X-100, and 2 mM each of EDTA and EGTA), and subjected to sonication on ice. Protein concentration was determined by the BioRad protein assay reagent (Hercules, CA). Equal amounts of proteins (250–500 µg) were loaded into respective ELISA wells for the assessment of IL-6 and IL-1β (Invitrogen, Carlsbad, CA). Serum was analyzed using a Bio-Plex Pro Mouse Cytokine Th17 Panel A -6-Plex kit (BioRad).
3
Multiplex Cytokine Analysis in Mouse Serum
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Blood was collected from sacrificed mice via inferior vena cava using a 22 G needle and transferred to serum‐gel Z tubes (Sarstedt, Germany), allowed to clot for 30 min at room temperature and centrifuged at 10 000 g for 5 min. The serum was collected and stored frozen until use. To detect interleukin‐1 beta (Il‐1β), interleukin‐6 (Il‐6), and tumour necrosis factor‐alpha (Tnf‐α) simultaneously, Bio‐Plex Pro™ mouse cytokine Th17 panel A 6‐Plex kit (Bio‐Rad, Germany) was used according to the manufacturer's instructions. Samples were diluted at 1:2, and the fluorescence measurement of the beads was performed with the Qiagen LiquiChip 200 workstation (Hilden, Germany). Cytokine concentrations were calculated using Bio‐Plex Manager software (Bio‐Rad, Hercules, CA, USA).
4
Urinary Biomarkers in Autoimmune Disease
5
Cytokine Profiling in Cardiomyocyte and Tissue
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Cardiomyocyte supernatant was analyzed by ELISA kits for interleukin (IL)-6 and tumor necrosis factor-α (TNF-a) (BD Biosciences, San Jose, CA) according to the manufacturer's instructions. Cardiac and lung tissue was crushed in liquid nitrogen, and equal weights of powdered tissues (~50 mg) were dissolved in 500 µl of lysis buffer (10 mM Hepes, pH 7.4, 5 mM MgCl 2 , 1 mM DTT, 1% Triton X-100, and 2 mM each of EDTA and EGTA), and subjected to sonication on ice. Protein concentration was determined by the BioRad protein assay reagent (Hercules, CA). Equal amounts of proteins (250-500 µg) were loaded into respective ELISA wells for the assessment of IL-6 and IL-1β (Invitrogen, Carlsbad, CA). Serum was analyzed using a Bio-Plex Pro Mouse Cytokine Th17 Panel A -6-Plex kit (BioRad).
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