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The NL20 cells are a human cell line derived from normal lung tissue. They are characterized as being non-tumorigenic and non-immortalized. The NL20 cells can be used for various in vitro studies, including those related to lung biology and disease.

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Lab products found in correlation

J774a 1 cells, by American Type Culture Collection (1 mentions) Celltiter aqueous one solution cell proliferation assay, by Promega (1 mentions) A549 cell, by American Type Culture Collection (1 mentions) Mrc 5, by American Type Culture Collection (1 mentions)

2 protocols using nl20 cells

1

Cytotoxicity Evaluation of Algae-NP-robot

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To evaluate cytotoxicity, J774A.1 cells (ATCC TIB-67) and NL20 cells (ATCC CRL-2503) were seeded in 96-well plates at 1× 104 cells per well. The cells were then incubated with algae-NP-robot with an algae/ cell ratio of 0.0625, 0.125, 0.25, 0.5, 1.0, 2.0, 4.0, 8.0, 16.0, 32.0, and 64.0 for 24 hours. CellTiter AQueous One Solution cell proliferation assay (Promega) was used to evaluate cell viability, according to the manufacturer’s instructions.
Zhang F., Zhuang J., Li Z., Gong H., Esteban-Fernández de Ávila B., Duan Y., Zhang Q., Zhou J., Yin L., Karshalev E., Gao W., Nizet V., Fang R.H., Zhang L, & Wang J. (2022). Nanoparticle-modified microrobots for in vivo antibiotic delivery to treat acute bacterial pneumonia. Nature materials, 21(11), 1324-1332.
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2

Culturing Lung Cell Lines

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A549 cells (the human lung alveolar epithelial cell line), NL‐20 cells (the human bronchial epithelial cell line), and MRC5 (the human lung fibroblast cell line) cells were purchased from ATCC and maintained in culture under standard conditions at 37°C and 5% CO2. Please see details in Supplementary Methods.
Zhu C., Potenza D.M., Yang Y., Ajalbert G., Mertz K.D., von Gunten S., Ming X, & Yang Z. (2023). Role of pulmonary epithelial arginase‐II in activation of fibroblasts and lung inflammaging. Aging Cell, 22(4), e13790.
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