We used the cornea neutralization technique [8 (link)] to visualize the rhabdomeres from the ommatidia of the fly compound eye. Briefly, dissected fly heads were mounted on a microscope slide using a drop of nail polish and further covered with oil. The number of ommatidia lacking rhabdomeres was counted using a Leica DMI4000B/DFC 340FX inverted microscope and a 40× oil immersion objective. At least 50 ommatidia per fly and 5 flies per genotype were examined.
Dmi4000b dfc 340fx
Manufactured by Leica
The DMI4000B/DFC 340FX is a microscope system designed for versatile imaging applications. It features a motorized inverted microscope and a high-resolution digital camera. The core function of this product is to enable researchers and scientists to capture detailed images and documents their observations.
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Lab products found in correlation
2 protocols using dmi4000b dfc 340fx
1
Visualizing Fly Compound Eye Rhabdomeres
2
Cornea Neutralization for Visualizing Fly Rhabdomeres
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We used the cornea neutralization technique20 (link) to visualize the rhabdomeres from the ommatidia of the fly compound eye. Briefly, dissected fly heads were mounted on microscope slides using nail polish and further covered with oil. The number of ommatidia lacking rhabdomeres was counted using a Leica DMI4000B/DFC 340FX inverted microscope and a 40 × oil immersion objective. At least 50 ommatidia per fly and 5 flies per genotype were examined blindly for each time point.
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