Hetastarch

Manufactured by B. Braun

Sourced in United Kingdom, United States

Hetastarch is a colloid solution that can be used as a plasma volume expander. It is a synthetic, hydroxyethylated starch that is designed to maintain blood volume and oncotic pressure. The core function of Hetastarch is to provide volume expansion and support circulatory function.

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Lab products found in correlation

Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions) Lidocaine, by B. Braun (1 mentions) Isoproterenol hcl, by Merck Group (1 mentions) 3 isobutyl 1 methylxanthine ibmx, by Merck Group (1 mentions) Forskolin, by Merck Group (1 mentions) Mrs1754, by Bio-Techne (1 mentions) Hespan, by B. Braun (1 mentions) Ficoll hypaque solution, by TBD Science (1 mentions) Pkh26gl, by Merck Group (1 mentions)

4 protocols using hetastarch

Redesigned IV Bag Label Evaluation

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The current label condition used 500-ml IV bags of lidocaine and hetastarch manufactured by B. Braun Medical Inc (Bethlehem, PA)—the labels involved in the close call that prompted this study (Fig. 1).²⁴The redesigned labels incorporated the 3 design recommendations under investigation and were developed using an iterative design process with feedback from pharmacists, anesthesiologists, and nurse anesthetist end users. The redesigned labels contained all of the same information as the current labels. The redesigned labels were printed on adhesive labels using a photo quality printer and affixed to unlabeled 500-ml IV bags (Fig. 2).

Estock J.L., Murray A.W., Mizah M.T., Mangione M.P., Goode JS J.r, & Eibling D.E. (2015). Label Design Affects Medication Safety in an Operating Room Crisis: A Controlled Simulation Study. Journal of Patient Safety, 14(2), 101-106.

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Synthesis and Characterization of ITI-214

Cited 1 time

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ITI-214 (full molecular weight (phosphate salt) = 606, 6aR,9aS)-2-(4-(6-fluoropyridin-2-yl)benzyl)-5-methyl-3-(phenyl-amino)-5,6a,7,8,9,9a-hexahydrocyclopenta-[4,5]imidazo[1,2-a]pyrazolo[4,3-e]pyrimidin-4-(2H)-one phosphate), was synthesized by Intra-Cellular Therapies, Inc. (New York, NY). Its K_i for full-length recombinant r-hPDE1A, 1B, and 1C is 34, 380, and 37 pM, respectively, with 900-fold greater activity toward PDE1C isoforms compared with the next nearest PDE family enzyme, PDE4D (K_i = 33 nM), and 10⁴-3×10⁵ -fold selectivity toward all other PDE enzyme families^{28 (link)}. Additional pharmaceuticals were dobutamine (Hospira Inc, Lake Forest, IL), esmolol HCl (Mylan, Rockford, IL), cilostamide, MRS1754 (Tocris Bioscience, Bristol, UK), Hespan (6% Hetastarch in 0.9% NaCl, B. Braun Medical Inc., Bethlehem, PA), forskolin, isoproterenol HCl, and 3-Isobutyl-1-methylxanthine (IBMX) (Sigma-Aldrich, St. Louis, MO).

Hashimoto T., Kim G.E., Tunin R.S., Adesiyun T., Hsu S., Nakagawa R., Zhu G., O’Brien J.J., Hendrick J.P., Davis R.E., Yao W., Beard D., Hoxie H.R., Wennogle L.P., Lee D.I, & Kass D.A. (2018). Acute Enhancement of Cardiac Function by Phosphodiesterase Type 1 Inhibition: A translational study in the dog and rabbit. Circulation, 138(18), 1974-1987.

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Neutrophil Isolation from Peripheral Blood

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Peripheral blood samples were bought from Tianjin Blood Center(China) with EDTA anticoagulation. Neutrophils were purified using a standard protocol. Firstly, erythrocytes were sedimented by adding an equal volume of hetastarch(B. Braun, US)at room temperature for 30 min. The erythrocyte-depleted supernatants were then layered on the lymphocyte separation medium(Ficoll-Hypaque solution, TBDScience) and centrifuged at 2000 rpm at room temperature for 20 min. Contaminated erythrocytes in the neutrophil pellets were lysed after a treatment with red blood lysis buffer(STEM CELL TM Technology, CA). Neutrophils were washed and resuspended in RPMI 1640 medium(Thermo Scientific, US) containing 10% Fetal Bovine Serum(Hyclone) at a density of 3×10⁶ cells per ml and maintained at 37°C.

Cao S., Liu P., Zhu H., Gong H., Yao J., Sun Y., Geng G., Wang T., Feng S., Han M., Zhou J, & Xu Y. (2015). Extracellular Acidification Acts as a Key Modulator of Neutrophil Apoptosis and Functions. PLoS ONE, 10(9), e0137221.

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Isolation and Labeling of PMNs and SS-RBCs

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HbSS-Townes mice were administered drinking water with or without 100 mM 2FF for 7 days. Heparinized whole blood was collected by heart puncture and PMNs and SS-RBCs were separated from mononuclear cells as previously described [20 (link)] on Histopaque 1077 (Corning Cellgro). The PMNs and SS-RBCs were separated by sedimentation of SS-RBCs on 6% hetastarch (B. Braun Medical) for 90 minutes at room temperature. The remaining SS-RBCs in the PMN fraction were lysed in ice cold water for 30 seconds. PMNs and sedimented SS-RBCs were washed twice in cell culture media containing RPMI 1640, 10% fetal bovine serum, 2 mM L-glutamine and 2 mM sodium pyruvate. Viability of the cell preparation was assessed using trypan blue dye exclusion and was always found to be in excess of 97%. PMNs were fluorescently labeled with 10 mM rhodamine 6G in PBS for 5 minutes at room temperature in the dark. SS-RBCs were fluorescently labeled for 5 minutes in the dark using a red fluorescent cell linker kit for general cell membrane labeling (Sigma-Aldrich #PKH26GL) according to the manufacturer’s protocol. After labeling, PMNs and SS-RBCs were washed 2 times in PBS.

Belcher J.D., Chen C., Nguyen J., Abdulla F., Nguyen P., Nguyen M., Okeley N.M., Benjamin D.R., Senter P.D, & Vercellotti G.M. (2015). The Fucosylation Inhibitor, 2-Fluorofucose, Inhibits Vaso-Occlusion, Leukocyte-Endothelium Interactions and NF-ĸB Activation in Transgenic Sickle Mice. PLoS ONE, 10(2), e0117772.

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