HEK293 cells and HEK293T cells were cultured in Dulbecco’s modified Eagle medium (DMEM, Gibco) containing 10 % fetal bovine serum (FBS, Gibco) and 1 % glutamine (Gibco). THP-1 cells and Jurkat cells were cultured in Roswell Park Memorial Institute medium (RPMI, Gibco) supplemented with 10 % FBS and 1 % glutamine. The cells were cultured at 37 °C with 5 % CO2.
The antibodies and reagents used in this work were as follows: anti-HA (Cell signaling technology,CST), anti-p24 (Santa Cruz Biotechnology), anti-TRIM11 (Sigma-Aldrich), anti-Myc (Roche); puromycin and MG132 (Sigma-Aldrich), chloroquine (CST), nocodazole (Beoytime) and taxol (Tocris Bioscience).
The antibodies and reagents used in this work were as follows: anti-HA (Cell signaling technology,CST), anti-p24 (Santa Cruz Biotechnology), anti-TRIM11 (Sigma-Aldrich), anti-Myc (Roche); puromycin and MG132 (Sigma-Aldrich), chloroquine (CST), nocodazole (Beoytime) and taxol (Tocris Bioscience).