Mouse lung and spleen single-cell suspensions were prepared and RBC were lysed using ACK buffer as previously described50 (link). RBC-free lung cells were washed with complete medium and labeled with bead-conjugated anti-CD11c (Miltenyi) to isolate lung APCs using autoMACS. RBC-free splenic cells were labeled with bead-conjugated anti-CD4 (Miltenyi) to isolate CD4+ T cells.
Bead conjugated anti cd11c
Manufactured by Miltenyi Biotec
Bead-conjugated anti-CD11c is a laboratory reagent used to identify and isolate cells expressing the CD11c antigen. It consists of magnetic beads coated with antibodies specific to the CD11c cell surface marker. This product can be used in various cell separation and analysis techniques.
Automatically generated - may contain errors
4 protocols using bead conjugated anti cd11c
1
Isolate Lung APCs and Splenic CD4+ T Cells
2
Isolation and Culture of Mouse BMDCs
3
Isolation and Culture of Mouse BMDCs
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Bone marrow cells isolated from eight-week old mice were lysed of RBC using ACK (Ammonium-Chloride-Potassium) after which cells were washed with complete medium (RPMI 1640 medium, supplemented with 10% Hyclone heat-inactivated FBS, GE Healthcare Life Sciences) and plated in 6-well plates at 5–10 × 105 cells/ml. The culture medium was supplemented with mouse GM-CSF (20 ng/ml) and IL-4 (10 ng/ml) (R&D Systems Inc.). On day 5 or 6, both adherent and non-adherent cells were harvested by gently scraping. CD11c+ BMDCs were isolated with bead-conjugated anti-CD11c (Miltenyi) using autoMACS.
4
Isolate Lung APCs and Splenic CD4+ T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse lung and spleen single-cell suspensions were prepared and RBC were lysed using ACK buffer as previously described50 (link). RBC-free lung cells were washed with complete medium and labeled with bead-conjugated anti-CD11c (Miltenyi) to isolate lung APCs using autoMACS. RBC-free splenic cells were labeled with bead-conjugated anti-CD4 (Miltenyi) to isolate CD4+ T cells.
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