Blood was collected at 1- to 3-month intervals beginning at 2 months of age via submandibular vein sampling of nonanesthetized mice. A terminal blood collection was performed via cardiac puncture of isoflurane-anesthetized mice. To assess parathyroid function, serum calcium analysis was performed using the Calcium LiquiColor assay (Stanbio/EKF Diagnostics, Boerne, TX, USA); PTH levels were measured using the Mouse Intact PTH ELISA Kit (Immutopics/Quidel, San Clemente, CA, USA; RRID:AB_2941776). To assess kidney function, blood urea nitrogen was measured using the Enzymatic Urea Nitrogen assay (Stanbio). Creatinine was measured using the Creatinine Serum Detection kit (Enzo Life Sciences, Farmingdale, NY, USA) and the Creatinine Detection Kit (Enzo) for urine. Liver function was assessed by measuring serum albumin levels using the Albumin LiquiColor assay (Stanbio).
Creatinine detection kit
Manufactured by Enzo Life Sciences
Sourced in United States
The Creatinine Detection Kit is a laboratory product designed to measure the concentration of creatinine in biological samples. Creatinine is a waste product formed by the breakdown of creatine, a compound found in muscle tissue. The kit provides a quantitative analysis of creatinine levels, which can be used to assess kidney function and other related health conditions.
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Lab products found in correlation
2 protocols using creatinine detection kit
1
Longitudinal Biomarker Assessment in Mice
2
Quantification of Oxidative Stress Markers
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This study used the following apparatus: microplate reader (SUNRISE; Serial number: 1001006648; Firmware: V 3.32 08/07/08; XFLUOR4 Version: V 4.51); Multi-mode Microplate Reader FlexStation® (Molecular Devices, USA); plate shaker (Heidolph Unimax 1010); microcentrifuge (Allegra 64 R); AfinionTM AS100 Analyzer (Abbott); and ultrafreezer -80°C (Biomedical, Lab Tech). Chemicals used in this study were creatinine detection kit (Enzo Life Sciences, Cat. No. ADI 907–030 A); 8-iso-PGF2α ELISA kit (Enzo Life Sciences, Cat. No. ADI-900-010); AtherOx® (oxLDL/β2GPI antigen complexes) kit (Corgenix, USA); and H2O2 reagents (xylenol orange, sorbitol, catalase enzyme, 30% H2O2, sulfonic acid, Fe(NH4)2(SO4)6H2O, NaH2PO4.H2O, Na2HPO42H2O, and deionized water).
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