Highly polymerized calf thymus DNA (∼10 000 base pairs, lyophylized powder) was purchased from Sigma (USA). DNA was dissolved in water that was first deionized and then destilled two times. DNA was then extensively dialyzed against buffer (20 mM phosphate, 1 mM EDTA, pH = 7) using a dialysis tube Float-A-Lyser (Spectrum Laboratories U.S., Mw cutoff 500 Da). The starting solution of DNA was heated to 95 °C in an outer thermostat and then cooled to 5 °C at cooling rate of 0.075 °C/min to allow DNA to adopt double-stranded DNA structure. Concentration of DNA base pairs in buffer solution was determined spectrophotomerically at 25 °C, λ = 260 nm, using molar extinction coefficient ε = 13200 M−1 cm−1.
Highly polymerized calf thymus dna
Manufactured by Merck Group
Sourced in United States
Highly polymerized calf thymus DNA is a laboratory product that consists of deoxyribonucleic acid (DNA) extracted from the thymus gland of calves. It is characterized by a high degree of polymerization, which means the DNA molecules have a large number of nucleotides in their backbone. This product is commonly used as a standard or reference material in various biochemical and molecular biology applications, such as nucleic acid research and analysis.
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2 protocols using highly polymerized calf thymus dna
1
DNA Preparation and Characterization
2
Characterization of Pt(II) Complexes
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Highly polymerized calf thymus
DNA was purchased from Sigma-Aldrich. All chemicals and solvents were
prepared by Merck (Germany). cis-[Pt(NH3)2I2] and [Pt(bipy)Cl2] were synthesized
using the methods described in the literature.52 (link)−55 (link) Infrared monitoring (4000–400
cm–1) was done on a JASCO-460 plus FT-IR spectrophotometer.
LC-MS analysis was performed on a Shimadzu LC-MS-2010 A system. A
Bruker BRX-250 Avance spectrometer was used at 300, 75, and 64 MHz
for 1H NMR, 13C NMR, and 195Pt NMR
monitoring, respectively. A Systronics Conductivity Bridge, model
305 (cell constant of 0.59), was used to measure the conductivities
of the two complexes in water. Melting points were recorded using
a Buchi B-545 system. A circular dichroism study was done using a
Jasco J-1500 CD spectrometer. The absorbance was recorded using a
SPEKOL 2000 UV 6800 spectrophotometer. The fluorescence intensity
was examined using a Hitachi MPF-4 spectrofluorimeter.
DNA was purchased from Sigma-Aldrich. All chemicals and solvents were
prepared by Merck (Germany). cis-[Pt(NH3)2I2] and [Pt(bipy)Cl2] were synthesized
using the methods described in the literature.52 (link)−55 (link) Infrared monitoring (4000–400
cm–1) was done on a JASCO-460 plus FT-IR spectrophotometer.
LC-MS analysis was performed on a Shimadzu LC-MS-2010 A system. A
Bruker BRX-250 Avance spectrometer was used at 300, 75, and 64 MHz
for 1H NMR, 13C NMR, and 195Pt NMR
monitoring, respectively. A Systronics Conductivity Bridge, model
305 (cell constant of 0.59), was used to measure the conductivities
of the two complexes in water. Melting points were recorded using
a Buchi B-545 system. A circular dichroism study was done using a
Jasco J-1500 CD spectrometer. The absorbance was recorded using a
SPEKOL 2000 UV 6800 spectrophotometer. The fluorescence intensity
was examined using a Hitachi MPF-4 spectrofluorimeter.
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