Cd4 bv605 and ccr7 pacific blue

Manufactured by BioLegend

CD4 BV605 is a fluorochrome-conjugated antibody that binds to the CD4 surface antigen, which is expressed on a subset of T cells. CCR7 Pacific blue is a fluorochrome-conjugated antibody that binds to the CCR7 chemokine receptor, which is involved in lymphocyte homing and trafficking.

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2 protocols using cd4 bv605 and ccr7 pacific blue

Multiparametric Flow Cytometry Analysis of PBMCs

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PBMCs were thawed, stained with 5 μl of the HLA-A*02:01 dextramer conjugated to PE for 10 minutes at room temperature, followed by the addition of 100 μl of a mastermix of anti-membrane marker mAbs containing LIVE/DEAD fixable aqua stain (Molecular Probes, Invitrogen), CD3 ECD (Beckman Coulter), CD4 BV605 and CCR7 Pacific blue (Biolegend), CD8 Alexa Fluor 700, CD14 PE-Cy7, CD16 PE-Cy7, CD19 PE-Cy7, CD45RA APC, CD57 FITC, TIGIT PerCP-eFluor710, PD-1 APC-eFluor780 (eBiosciences) and CD27 Qdot 655 (Life Technologies). The cells were incubated at 4°C for a further 20 minutes, washed and fixed with 1% paraformaldehyde in PBS prior to running on an LSRII flow cytometer (Becton-Dickinson).

Moyo N., Borthwick N.J., Wee E.G., Capucci S., Crook A., Dorrell L, & Hanke T. (2017). Long-term follow up of human T-cell responses to conserved HIV-1 regions elicited by DNA/simian adenovirus/MVA vaccine regimens. PLoS ONE, 12(7), e0181382.

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Comprehensive Immune Phenotyping of PBMCs

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PBMCs were thawed, incubated with 5 μl of the HLA-A*02:01/YQYMDDLV tetramer (NIH Tetramer Core Facility) conjugated to PE for 10 min at room temperature, followed by the addition of 100 μl of a mastermix of anti-membrane marker mAbs containing LIVE/DEAD fixable aqua stain (Molecular Probes, Invitrogen), CD3 ECD (Beckman Coulter), CD4 BV605 and CCR7 Pacific blue (Biolegend), CD8 Alexa Fluor 700, CD14 PE-Cy7, CD16 PE-Cy7, CD19 PE-Cy7, CD45RA APC, CD57 FITC, TIGIT PerCP-eFluor710, PD-1 APC-eFluor780 (eBiosciences) and CD27 Qdot 655 (Life Technologies). The cells were incubated at 4°C for a further 20 min, washed and fixed with 1% paraformaldehyde in PBS prior to running on an LSRII flow cytometer (Becton-Dickinson).

Borthwick N.J., Lane T., Moyo N., Crook A., Shim J.M., Baines I., Wee E.G., Hawkins P.N., Gillmore J.D., Hanke T, & Pepys M.B. (2018). Randomized phase I trial HIV-CORE 003: Depletion of serum amyloid P component and immunogenicity of DNA vaccination against HIV-1. PLoS ONE, 13(5), e0197299.

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