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Single cell 3 mrna kit

Manufactured by 10x Genomics
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The Single-cell 3' mRNA kit by 10x Genomics is a laboratory equipment product designed for the analysis of single-cell gene expression. The kit provides a method for capturing and sequencing the 3' end of mRNA transcripts from individual cells, allowing for the study of transcriptional profiles at the single-cell level.

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Lab products found in correlation

Novaseq, by Illumina (2 mentions) Automated cell counter, by Countstar (2 mentions)

2 protocols using single cell 3 mrna kit

1

Single-cell RNA-seq library preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sorted cells were washed and resuspended in 0.04% BSA/PBS buffer. Cells were counted on a Countess II automated cell counter and were loaded on a 10X Chromium chip with a target cell recovery of 6,000 cells per lane. Sequencing libraries were performed using the single-cell 3’ mRNA kit according to the manufacturer’s protocol (10X Genomics). cDNA and library quality were examined on an Agilent 4200 TapeStation and quantified by qPCR (Kapa Biosystems / Roche). Illumina sequencing (NovaSeq) was performed using a paired-end 100bp protocol. Libraries were sequenced to a median depth of 50,000 unique reads per cell.
Johnson K.C., Anderson K.J., Courtois E.T., Gujar A.D., Barthel F.P., Varn F.S., Luo D., Seignon M., Yi E., Kim H., Estecio M.R., Zhao D., Tang M., Navin N.E., Maurya R., Ngan C.Y., Verburg N., de Witt Hamer P.C., Bulsara K., Samuels M.L., Das S., Robson P, & Verhaak R.G. (2021). Single-cell multimodal glioma analyses identify epigenetic regulators of cellular plasticity and environmental stress response. Nature genetics, 53(10), 1456-1468.
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2

Single-cell RNA-seq library preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Sorted cells were washed and resuspended in 0.04% BSA/PBS buffer. Cells were counted on a Countess II automated cell counter and were loaded on a 10X Chromium chip with a target cell recovery of 6,000 cells per lane. Sequencing libraries were performed using the single-cell 3’ mRNA kit according to the manufacturer’s protocol (10X Genomics). cDNA and library quality were examined on an Agilent 4200 TapeStation and quantified by qPCR (Kapa Biosystems / Roche). Illumina sequencing (NovaSeq) was performed using a paired-end 100bp protocol. Libraries were sequenced to a median depth of 50,000 unique reads per cell.
Johnson K.C., Anderson K.J., Courtois E.T., Gujar A.D., Barthel F.P., Varn F.S., Luo D., Seignon M., Yi E., Kim H., Estecio M.R., Zhao D., Tang M., Navin N.E., Maurya R., Ngan C.Y., Verburg N., de Witt Hamer P.C., Bulsara K., Samuels M.L., Das S., Robson P, & Verhaak R.G. (2021). Single-cell multimodal glioma analyses identify epigenetic regulators of cellular plasticity and environmental stress response. Nature genetics, 53(10), 1456-1468.
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