Triglycerides glycerol blanked

Participants underwent blood sampling for the assessment of blood chemistry and hormonal parameters. The quantitative measurement of irisin in human serum samples was performed using a commercial enzyme linked immunosorbent assay (ELISA) kit (Irisin ELISA Kit EK-067-52; Phoenix Pharmaceuticals Inc., CA) according to the manufacturer’s instructions. Basal insulin was analyzed by an immunoradiometric assay (BioSource International, Camarillo, CA, USA) in a Beckman Coulter (Fullerton, CA, USA). Enzymatic colorimetric assay was used to measure fasting plasma glucose (FPG) (Hexokinase activity assay, Abcam), total cholesterol (Cholesterol Gen.2, Roche), high-density lipoproteins (HDL) cholesterol (LDL-Cholesterol plus 2nd generation, Roche), triglycerides (Triglycerides/Glycerol Blanked, Roche) concentrations using a Dimension Autoanalyzer (Cobas501, Roche, Switzerland). The homeostatic model assessment index was calculated following the formulas: HOMA-IR = fasting serum insulin (FINS, mU/L) × fasting plasma glucose (FPG, mmol/L)/22.5; HOMA-IS = 1/(FPG (mmol/L) × FINS (mIU/L)). Low-density lipoprotein (LDL) cholesterol serum concentration was calculated with Friedewald’s formula.