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Revertra ace qpcr rt master

Manufactured by Toyobo
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Sourced in Japan

ReverTra Ace qPCR RT master is a laboratory reagent used for reverse transcription and real-time quantitative PCR (qPCR) applications. It enables the conversion of RNA into complementary DNA (cDNA) and subsequent amplification and quantification of target DNA sequences.

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Lab products found in correlation

Kapa sybr fast qpcr kit, by Roche (1 mentions) Rnaiso plus, by Takara Bio (1 mentions) Gdna remover, by Toyobo (1 mentions) Steponeplus real time pcr system, by Thermo Fisher Scientific (1 mentions) Gdna remover kit, by Toyobo (1 mentions)

Spelling variants of this product

ReverTra Ace (1031 citations) ReverTra AceTM qPCR RT Master Mix (13 citations) ReverTra AceTM qPCR RT Master Mix with gDNA Remover (11 citations)

2 protocols using revertra ace qpcr rt master

1

Transcriptome Analysis of PDLF Cells

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Total RNA from PDLF was purified using RNAiso plus (Takara Bio Inc., Otsu, Japan). Using the ReverTra Ace qPCR RT master mix with gDNA remover (Toyobo Life Science, Tokyo, Japan), 0.5 μg of total RNA was reverse transcribed into cDNA using mixed primers. qPCR reactions were prepared using the KAPA SYBR Fast qPCR kit (KAPA BIOSYSTEMS, Woburn, MA, USA). Human HPRT was used as an internal reference control. PCR primer sequences for target genes are shown in Table A1.
Sato A., Suzuki S., Yuan H., Fahreza R.R., Wang X., Nemoto E., Saito M, & Yamada S. (2023). Pharmacological Activation of YAP/TAZ by Targeting LATS1/2 Enhances Periodontal Tissue Regeneration in a Murine Model. International Journal of Molecular Sciences, 24(2), 970.
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2

Quantification of Mouse and Human mRNAs

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Total cellular RNA (0.5 µg), isolated with Isogen II (Toyobo), was reverse-transcribed with ReverTra Ace qPCR RT Master Mix with a gDNA Remover kit (Toyobo). Quantitative PCR (StepOnePlus Real-Time PCR system; Life Technologies) was performed for quantitation of various mouse and human mRNAs. Mouse or human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used for normalization of the results. The sequences of the primers used for amplification are available upon request.
Tanaka S., Maekawa A., Matsubara L., Imanishi A., Yano M., Roeder R.G., Hasegawa N., Asano S, & Ito M. (2016). Periostin supports hematopoietic progenitor cells and niche-dependent myeloblastoma cells in vitro. Biochemical and biophysical research communications, 478(4), 1706-1712.
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