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Aunp 12

Manufactured by Selleck Chemicals
Sourced in United States

The AUNP-12 is a laboratory equipment designed for the synthesis and characterization of gold nanoparticles. It features precise temperature control, stirring, and monitoring capabilities to facilitate the production of gold nanoparticles with consistent size and morphology.

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2 protocols using aunp 12

1

Plasmid-based PTEN Gene Therapy

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The three plasmid systems, including target gene plasmid (pAAV-CAG-mCherry), capsid plasmid (pAAV-6, pAAV-1, pAAV-7m8, pAAV-7, pAAV-8, pAAV-10) and helper plasmid (pHelper), were all purchased from Fenghui Biotechnology (Hunan, China). The complementary DNA (cDNA) encoding the mouse PTEN gene was synthesized from GENEWIZ (Suzhou, China). pAAV-CAG-PTEN was obtained by replacing mCherry gene in pAAV-CAG-mCherry with PTEN gene. Reverse the PTEN gene sequence in pAAV-CAG-PTEN to get pAAV-CAG-PTENR.
The anti-PD-1 used in this study is AUNP-12, a polypeptide purchased from Selleck (USA) with a purity of 99.20%. Cy5-AUNP-12 was purchased from Guoping pharmaceutical co (Anhui, China) with a purity of 90%. CpG was synthesized in GENEWIZ (Suzhou, China) with 90% purity.
Antibodies for Western blot (WB) and Immunofluorescence (IF): anti-mouse PTEN, CRT, β-actin and anti-rabbit FITC, CY3 fluorescent secondary antibodies were purchased from ABclonal Tech (Wuhan, China). Antibodies for flow cytometry test: CD11c-PE, CD80-FITC, CD86-APC, CD3-APC, CD8-FITC, CD4-APC-eflour780, CD69-PE, CD274-PE, CD11b-FITC, CD206-PE-Cy7, CD86-APC, IL-4-PE, IFN-γ-APC, TNF-α-eflour450, IL-2-PE5.5, CD4-APC-eflour780, CD44-APC, CD62L-PE were all purchased from Invitrogen (USA).
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2

Synthesis and Characterization of Bioactive Compounds

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CA-170 was purchased from MedChemExpress (Monmouth Junction, NJ, USA) Cas: 1673534-76-3, AUNP-12 was purchased from SelleckChem (Houston, TX, USA) BMS-1166 was synthesized according to the protocol from described in [22 (link)], peptide-57 was synthesized as described in [49 (link)].
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