A Nikon Ti-E inverted fluorescence microscope (Nikon Instruments, Japan) with a 100X NA 1.40 oil immersion phase contrast objective (Nikon Instruments, Japan) was used for imaging the bacteria. Images were captured on an iXon DU897 EMCCD camera (Andor Technology, Ireland) and recorded using NIS-Elements software (Nikon Instruments, Japan). Fluorophores were excited by a 200W Hg lamp through ND4 and ND8 neutral density filters. Chroma 41004 and 41001 filter cubes (Chroma Technology Corp., VT) were used to record mCherry and Ypet images, respectively. A motorized stage (Prior Scientific Inc., MA) and a Nikon Perfect Focus ® system were utilized throughout time-lapse imaging.
Chroma 41004 and 41001 filter cubes
Manufactured by Chroma Technology
The Chroma 41004 and 41001 filter cubes are optical components designed for use in various laboratory and research applications. The 41004 model is a four-position filter cube, while the 41001 is a single-position filter cube. These filter cubes are used to house and position optical filters, which are essential for filtering and selecting specific wavelengths of light. The core function of these products is to provide a consistent and reliable way to incorporate filters into optical systems, enabling researchers and scientists to conduct their experiments and analyses effectively.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using chroma 41004 and 41001 filter cubes
1
Fluorescence Microscopy of Bacteria
2
Fluorescence Microscopy of Bacteria
Check if the same lab product or an alternative is used in the 5 most similar protocols
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A Nikon Ti-E inverted fluorescence microscope (Nikon Instruments, Japan) with a 100X NA 1.40 oil immersion phase contrast objective (Nikon Instruments, Japan) was used for imaging the bacteria. Images were captured on an iXon DU897 EMCCD camera (Andor Technology, Ireland) and recorded using NIS-Elements software (Nikon Instruments, Japan). Fluorophores were excited by a 200W Hg lamp through ND4 and ND8 neutral density filters. Chroma 41004 and 41001 filter cubes (Chroma Technology Corp., VT) were used to record mCherry and Ypet images, respectively. A motorized stage (Prior Scientific Inc., MA) and a Nikon Perfect Focus ® system were utilized throughout time-lapse imaging.
3
Fluorescence Microscopy of Bacteria
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A Nikon Ti-E inverted fluorescence microscope (Nikon Instruments, Japan) with a 100X NA 1.40 oil immersion phase contrast objective (Nikon Instruments, Japan), was used for imaging the bacteria. Images were captured on an iXon DU897 EMCCD camera (Andor Technology, Ireland) and recorded using NIS-Elements software (Nikon Instruments, Japan). Fluorophores were excited by a 200W Hg lamp through an ND4 and ND8 neutral density filter. Chroma 41004 and 41001 filter cubes (Chroma Technology Corp., VT)
were used to record mCherry and Ypet images, respectively. A motorized stage (Prior Scientific Inc., MA) and a Nikon Perfect Focus ® system were utilized throughout time-lapse imaging.
were used to record mCherry and Ypet images, respectively. A motorized stage (Prior Scientific Inc., MA) and a Nikon Perfect Focus ® system were utilized throughout time-lapse imaging.
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