P ire1α ab243665

Manufactured by Abcam

Sourced in United States

P-IRE1α (ab243665) is a recombinant antibody that recognizes the phosphorylated form of the Inositol-Requiring Enzyme 1-alpha (IRE1α) protein. IRE1α is a serine/threonine-protein kinase and endoribonuclease that plays a role in the unfolded protein response pathway.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using p ire1α ab243665

Elucidating ER Stress Pathway Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

PERK (#5683), eIF2α (#5324), p-eIF2α (#9721), CHOP (#2895), IRE1α (#3294), XBP1s (#27901), GRP78 (#3177), cleaved PARP (#9532), Bax (#2772), Bcl-2 (#2872) and β-actin (#9562) primary antibodies were purchased from Cell Signaling Technology (CST, MA, US). p-PERK (DF7576) antibody was purchased from Affinity Biosciences (Cincinnati, OH, USA). ATF6 (sc-166659) antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA, US). ATF4 (ab184909) and p-IRE1α (ab243665) were purchased from Abcam (Burlingame, CA, US). UBA1 inhibitor TAK-243 was obtained from CSNpharm (CSNpharm, Chicago, IL, US). GRP78 inhibitor HA15 and PERK inhibitor GSK2606414 were purchased from MedChemExpress (Shanghai, China). TAK-243, HA15 and GSK2606414 were dissolved in DMSO to create a 10 mmol/L solution, which was diluted to different concentrations in DMEM medium before use.

Zhang X., Wu R., Tian C., Wang W., Zhou L., Guo T., Yu J., Wu C., Shen Y., Liu X, & Yu R. (2022). GRP78 blockade overcomes intrinsic resistance to UBA1 inhibitor TAK-243 in glioblastoma. Cell Death Discovery, 8, 133.

+ Open protocol

+ Expand

Exploring Cellular Stress Response Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ubiquitin (#3933), UBA1 (#4891), p21 (#2947), Cyclin D1 (#2922), CDC2 (#9116), c-Jun (#9165), cleaved PARP (#9532), LC3B (#3868), PERK (#5683), p-PERK (#3179), CHOP (#2895), ATF4 (#11815), IRE1α (#3294), eIF2α (#5324), p-eIF2α (#9721), XBP1s (#27901), GRP78 (#3177), cleaved caspase-3 (#9661), p-JNK (#9251), and β-actin (#9562) primary antibodies were purchased from Cell Signaling Technology (CST, MA, USA). Antibody against Ki-67 (RM-9106) was purchased from Thermo Fisher (Waltham, MA, USA). ATF6 (sc-166659 and JNK1 (sc-1648) antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). p-IRE1α (ab243665) and p62 (ab56416) were purchased from Abcam (Burlingame, CA, USA). UBA1 inhibitor TAK-243 was obtained from CSNpharm (CSNpharm, Chicago, IL, USA). TAK-243 was dissolved in DMSO to create a 10 mmol/L solution, which was diluted to different concentrations in DMEM medium before use.

Liu G., Yu J., Wu R., Shi L., Zhang X., Zhang W., Zhong X., Wang Y., Li H., Shen Y., Wu C., Yu R., Niu M, & Liu X. (2021). GRP78 determines glioblastoma sensitivity to UBA1 inhibition-induced UPR signaling and cell death. Cell Death & Disease, 12(8), 733.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!