Total proteins were isolated by RIPA Lysis Buffer (Beyotime, Shanghai, China). Proteins concentrations were tested by bicinchoninic acid kit (Beyotime). The protein sample was separated on SDS-PAGE gel, transferred to polyvinylidene fluoride membranes, and followed by the blockage with 5% nonfat milk for 1 h. Next, the membranes were probed with primary antibodies of ZDHHC5 (1:1000, Proteintech), Bcl-2 (1:1000, Abcam), COX-2 (1:1000, Abcam), and GAPDH (1:1000, Beyotime) overnight at 4 °C. Then, membranes were incubated with secondary antibody (1:1000, Beyotime) for 2 h in a dark room at room temperature. GAPDH was used as the control protein. Enhanced chemiluminescence Plus reagent (Beyotime) was used to image blots. The band quantification was performed using Image J software.
Zdhhc5
Manufactured by Proteintech
ZDHHC5 is a protein-coding gene that belongs to the DHHC-containing palmitoyltransferase family. The ZDHHC5 enzyme catalyzes the attachment of palmitate, a type of fatty acid, to specific cysteine residues of target proteins in a process known as protein palmitoylation.
Automatically generated - may contain errors
Lab products found in correlation
Ripa lysis buffer, by Beyotime (2 mentions)
Bcl 2, by Abcam (2 mentions)
Cox 2, by Abcam (2 mentions)
Gapdh, by Beyotime (2 mentions)
Bicinchoninic acid kit, by Beyotime (1 mentions)
Enhanced chemiluminescence plus reagent, by Beyotime (1 mentions)
Cleaved caspase 3, by Abcam (1 mentions)
Bca kit, by Beyotime (1 mentions)
Ecl plus reagent, by Beyotime (1 mentions)
Bcl2 associated x (bax), by Abcam (1 mentions)
Pro caspase 3, by Abcam (1 mentions)
Pro caspase 9, by Abcam (1 mentions)
Cleaved caspase 9, by Abcam (1 mentions)
2 protocols using zdhhc5
1
Profiling Protein Expression Patterns
2
Protein Expression Analysis via Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Total proteins were isolated by RIPA Lysis Buffer (Beyotime, Shanghai, China). Proteins’ concentrations were tested by bicinchoninic acid (BCA) kit (Beyotime). The protein sample was separated on SDS/PAGE gel, and transferred to polyvinylidene fluoride membranes, followed by the blockage with 5% nonfat milk for 1 h. Next, the membranes were probed with primary antibodies of ZDHHC5 (1:1000, Proteintech), Bcl-2 (1:1000, Abcam), Bax (1:1000, Abcam), pro-caspase-3 (1:1000, Abcam), cleaved-caspase-3 (1:1000, Abcam), pro-caspase-9 (1:1000, Abcam), cleaved-caspase-9 (1:1000, Abcam), COX-2 (1:1000, Abcam), and GAPDH (1:1000, Beyotime) overnight at 4°C. Then, membranes were incubated with secondary antibody (1:1000, Beyotime) for 2 h keeping in a dark place at room temperature. The protein levels were detected by enhanced chemiluminescence (ECL) Plus reagent (Beyotime).
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