Cd3 cd19 cd56 apc

Manufactured by BD

Sourced in United States

CD3/CD19/CD56-APC is a fluorescent-labeled antibody panel that can be used to identify and enumerate T cells (CD3+), B cells (CD19+), and natural killer cells (CD56+) in biological samples. It provides a standardized method for the detection and quantification of these cell populations using flow cytometry.

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Lab products found in correlation

Cd45 apc cy7, by BD (2 mentions) Cd33 pe, by BD (2 mentions) Cd15 fitc, by BD (2 mentions) Facscanto flow cytometer, by BD (1 mentions) Ccr7 apc, by BD (1 mentions) Cd11c apc, by BD (1 mentions) Cd54 apc, by BD (1 mentions) Hla dr fitc, by BD (1 mentions) Cd80 fitc, by BD (1 mentions) Cd86 fitc, by BD (1 mentions) Cd123 pe, by BD (1 mentions) Cd14 apc, by BD (1 mentions) Cd38 fitc, by BD (1 mentions)

Close spelling variants of this product

CD3-APC (117 citations) CD19-APC (88 citations) CD56-APC (59 citations) CD3/CD19 (3 citations) BD Multitest™ CD3-FITC/CD16-PE+CD56-PE/CD45-PerCP/CD19-APC reagent (3 citations)

2 protocols using cd3 cd19 cd56 apc

Phenotypic Characterization of Monocyte-Derived Dendritic Cells

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Qualitative determinations of specific sub-populations were performed using fluorescent-labeled antibodies and flow cytometry. The purity of the elutriated monocytes was evaluated by flow cytometry using CD33-PE, CD15-FITC, CD3/CD19/CD56-APC and CD45-APC-Cy7 (Becton Dickinson, Mountain View, CA) and isotype controls (Becton Dickinson). The analysis of mDCs was undertaken after harvest on day 4. This included the standard “DC panel” adopted in our institution as lot release for mDCs products and other investigational markers. The panel consisted of CD86-FITC, CD83-PE, CD14-APC, CD209-FITC, CCR7-PE, CD40-APC, HLA-DRFITC, CD123-PE, CD11c-APC, CD80-FITC, CD154-PE, CD54-APC, CD16-FITC, CCR7-PE and CD1a-APC. The expression of HER2/neu was assessed by flow cytometry with anti-CD340 antibodies (BioLegend, San Diego, CA). Flow cytometry acquisition and analysis were performed with FACScanto flow cytometer (Becton Dickinson and Company, Franklin Lakes, NJ) according to CPS procedures. Spectral overlap was electronically compensated using single color controls. Quality controls were run before each session according to internal quality control policy.

Jin P., Chen W., Ren J., Chen S., Wood L., Zhao Y., Remaley A., Pham C., Lian S., Liu S., Liu H., Highfill S., Berzofsky J.A, & Stroncek D. (2018). Plasma from Some Cancer Patients Inhibits Adenoviral Ad5f35 Vector Transduction of Dendritic Cells. Cytotherapy, 20(5), 728-739.

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Comprehensive Immunophenotyping of Elutriated Monocytes and Dendritic Cells

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Analysis of expression of surface markers was performed using
fluorescent labeled antibodies (Abs) and flow cytometry. The purity of the
elutriated monocytes was assessed by flow cytometry using CD33-PE, CD15-FITC,
CD3/CD19/CD56-APC and CD45-APC-Cy7 (Becton Dickinson, Mountain View, CA, USA)
and isotype controls (Becton Dickinson). DC were analyzed after pulsing on Day
4. The analysis included the standard “DC panel” adopted in our
institution as lot release for mature DC products and other investigational
markers. The panel consisted of CD86-FITC, CD83-PE, CD14-APC, HLA-DR-FITC,
CD123-PE, CD11c-APC, CD80-FITC, CD54-APC, CCR7-APC, and CD38-FITC (Becton
Dickinson). Flow cytometry acquisition and analysis were performed with a
FACScanto flow cytometer (Becton, Dickinson and Company, Franklin Lakes, NJ USA)
according to CPS standard operating procedures. Spectral overlaps were
electronically compensated using single color controls. Quality controls were
run before each session according to internal quality control policy.

Castiello L., Sabatino M., Ren J., Terabe M., Khuu H., Wood L.V., Berzofsky J.A, & Stroncek D.F. (2017). Expression of CD14, IL-10 and tolerogenic signature in dendritic cells inversely correlate with clinical and immunological response to TARP vaccination in prostate cancer patients. Clinical cancer research : an official journal of the American Association for Cancer Research, 23(13), 3352-3364.

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