Mouse anti coilin

Manufactured by Merck Group

Mouse anti-Coilin is a laboratory reagent used for the detection and analysis of the Coilin protein in biological samples. Coilin is a marker for Cajal bodies, which are nuclear suborganelles involved in the assembly and modification of small nuclear ribonucleoproteins (snRNPs). The mouse anti-Coilin antibody can be used in various immunoassay techniques to identify and quantify the presence of Coilin in cells and tissues.

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Lab products found in correlation

2 protocols using mouse anti coilin

Antibody Characterization for Cell Biology

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The following are other antibodies used in this study: anti-actin-HRP (horseradish peroxidase) (Santa Cruz, sc-47778), mouse anti-PCNA (Abcam, ab29), rabbit anti-XPO1-C-terminal (Santa Cruz, sc-5595), mouse anti-XPO1-N-terminal (Santa Cruz, sc-136220), mouse anti-Coilin (Sigma C-1862), mouse anti-XPO5 (Abcam ab57491), mouse anti-XPOT (Abcam ab49933), rabbit anti-H3 (Abcam ab1791), mouse anti-GEMIN5 (Santa Cruz, sc-136200), mouse anti-SMN1 (Abcam, ab5831), goat anti-LaminB (Santa Cruz, sc-6216), mouse anti-CSE1L (Santa Cruz sc-135855), rabbit anti-Dyskerin (Santa Cruz sc-48794), rabbit anti-PHAX (Bethyl, A303–916A), goat anti-PHAX (M-19) (Santa Cruz, sc-11704), mouse anti-UBF1 (Santa Cruz sc-13125), mouse anti-nucleolin (Enzo, ADI-KAM-CP100) mouse anti-TRF2 (Imgenex, IG124A), mouse anti-XPO5 (Abcam ab57491), mouse anti-NFKB (Santa Cruz sc-372), mouse anti-RANBP2 (Santa Cruz, sc-74518), mouse anti-Fibrillarin (Abcam, ab4566), mouse anti-FLAG M2 (Sigma), IgG mouse (Santa Cruz sc-2025), mouse anti-Coilin (Sigma, C1862), rabbit anti-TCAB1 (Novus, NB100–68252); IgG rabbit (Abcam ab37415). All Alexa-conjugated secondary antibodies were purchased from Life Technologies.

Schertzer M., Jouravleva K., Perderiset M., Dingli F., Loew D., Le Guen T., Bardoni B., de Villartay J.P., Revy P, & Londoño-Vallejo A. (2015). Human regulator of telomere elongation helicase 1 (RTEL1) is required for the nuclear and cytoplasmic trafficking of pre-U2 RNA. Nucleic Acids Research, 43(3), 1834-1847.

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RNA ISH for CCAT1 Isoform Detection

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To detect CCAT1-L and CCAT1-S, RNA ISH was carried out as previously described with in vitro transcribed digoxigenin-labeled antisense probes^{32 (link)}. For colocalization studies, cells were co-stained with rabbit anti-Nucleolin (Santa Cruz Biotechnology), mouse anti-p54^nrb (BD) and mouse anti-Coilin (Sigma). The nuclei were counterstained with DAPI. Images were taken with a Zeiss LSM 510 microscope or with an Olympus IX70 DeltaVision RT Deconvolution System microscope.

Xiang J.F., Yin Q.F., Chen T., Zhang Y., Zhang X.O., Wu Z., Zhang S., Wang H.B., Ge J., Lu X., Yang L, & Chen L.L. (2014). Human colorectal cancer-specific CCAT1-L lncRNA regulates long-range chromatin interactions at the MYC locus. Cell Research, 24(5), 513-531.

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