Human breast epithelial adenocarcinoma cell lines (MCF‐7, MDA‐MB231, MDA‐MB468) were purchased from Iranian Biological Resource Center (IBRC). They were cultured in DMEM—Dulbecco’s Modified Eagle Medium (GIBCO,USA) supplemented with 10% heat inactivated foetal bovine serum (FBS; Invitrogen), 1% non‐essential amino acid, 2 mmol/L L‐glutamine, and 1% penicillin/streptomycin at 37° C in a humidified atmosphere with 5% CO2.
Mda mb 468
Manufactured by Iranian Biological Resource Center
MDA-MB-468 is a cell line derived from a human breast adenocarcinoma. It is a commonly used model in cancer research to study the biology and behavior of triple-negative breast cancer.
Automatically generated - may contain errors
Lab products found in correlation
Mcf 7, by Iranian Biological Resource Center (3 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Mda mb 231, by Iranian Biological Resource Center (1 mentions)
Sildenafil, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Caspase 3 antibody, by Cell Signaling Technology (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Triton x 100, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
3 protocols using mda mb 468
1
Cultivation of Breast Cancer Cell Lines
2
Sildenafil Effects on Breast Cancer Cells
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Chemical reagents, including Sildenafil, 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Triton X-100, and DMSO were provided (Sigma-Aldrich, St. Louis, MO, USA). Cell lines "MCF-7 and MDA-MB-468" were obtained from Iranian Biological Resource Center (Tehran, Iran). The caspase3 antibody was provided from Cell Signaling (cat# 9662S). High-glucose Dulbecco's Modified Eagle's Medium (EMEM), penicillin-streptomycin, and fetal bovine serum were purchased (Gibco, Germany).
3
Synergistic Anti-Cancer Effect of Sildenafil and Cisplatin
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Cell lines, including MCF-7 and MDA-MB-468 were provided from Iranian Biological Resource Center (Tehran, Iran). Cells culture were performed in DMEM medium containing fetal bovine serum (10%), 2 mM Lglutamine, 0.01 mg/mL bovine insulin (90%) and also Earle's BSS containing 1 mM sodium pyruvate, 1.5 g/L sodium bicarbonate, and 0.1 mM nonessential amino acids. Cells were treated by different concentration of drugs used 24 and 72 h as incubation times. For MTT and apoptosis assay, cells were seeded at 7000 and 100,000 per well, respectively. To validate the results precisely, there was a sample as a control. Different treatment was done in triplicate. Of note, cells were treated with sildenafil as a PDE5 inhibitor in combination with cisplatin to investigate the synergistic effect.
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