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5 protocols using carrageenan

1

Morroniside-Aspirin Interaction Study

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Morroniside standards (20 mg, Shanghai Shifeng Biotechnology Co., Ltd., batch number: 20140419, purity ≥98%); Aspirin enteric-coated tablets (Shiyao group Ouyi Pharmaceutical Co., Ltd); Ethanol absolute (Tianjin Beilian Fine Chemicals Development Co., Ltd); Acetic acid (Tianjin Taixing Reagent Factory); 37–40% formaldehyde solution (Tianjin Best Chemical Co., Ltd.); Carrageenan (Shanghai McLean biochemical Technology Co., Ltd.); Xylene (Tianjin Baishi Chemical Co., Ltd.).
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2

Crayfish Antimicrobial Assessment

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Live crayfish with an average weight of 30 ± 2.4 g and an average length of 20 ± 1.8 cm were purchased from Qianjiang Changgui Aquatic Food Co., Ltd. (Nantong, China). Four strains of bacteria, including Escherichia coli (AB93154), Shewanella putrefaciens (ATCCBAA-1097), Staphylococcus aureus (ATCC43979), and Salmonella typhimurium (ATBA45479), were purchased from the Shanghai Preservation Biotechnology Centre, (Shanghai, China). All other reagents (chitosan, lysozyme, carrageenan, MgO, KBr, trichloroacetic acid, etc.) used in this study were analytically pure and were purchased from McLean Biochemical Technology Co. Ltd. (Shanghai, China).
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3

Inflammatory Response Assay Protocol

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The ELISA kits of IL-6, IL-1β, TNF-α, and PGE2 were purchased from Shanghai Enzyme-linked Biotechnology Co., Ltd. (Shanghai, China). Dexamethasone was purchased from Shanghai Shangyao Xinyi Pharmaceutical Factory Co., Ltd. (Shanghai, China). Carrageenan and aspirin were purchased from Macklin Reagent Co., Ltd. (Shanghai, China). Xylene was obtained from Hangzhou Shuanglin Chemical Co., Ltd. (Hangzhou, China). Ammonia was purchased from Hangzhou Longshan Fine Chemical Co., Ltd. (Hangzhou, China). Citrate buffer solution and DAB were obtained from Beyotime Biotechnology Reagent Co., Ltd. (Zhejiang, China). BSA and anti-rabbit IgG were obtained from Boster Biological Technology Co., Ltd. (Zhejiang, China). COX-2, NF-κB p65, and GAPDH antibodies were purchased from Proteintech Biotechnology Co., Ltd. (Zhejiang, China).
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4

Rat MCAO Stroke Model Protocol

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Carrageenan was supplied by Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China, Batch No: C14408398). Active dry yeast was purchased from Angel Yeast Co., Ltd. (Yichang, China; Batch No: HY2009R). The rat Interleukin-6 (IL-6) Enzyme-Linked Immunosorbent Assay (ELISA) kit was acquired from Cohesion Biosciences. (UK; Batch No: CEK1619). Nylon suture for Middle Cerebral Artery Occlusion (MCAO) surgery was purchased from Hebei Tiannong Biotechnology Co., Ltd. (Shijiazhuang, China; Batch No: 20210630).
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5

Anti-inflammatory Mechanisms in Carrageenan-Induced Mice

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Male KM mice, weighing
16–18 g, were purchased from the Animal Laboratory Centre of
Jinzhou Medical University (Animal license number: SCXK [Liao] 2019-0007).
YXB (NO: 20210303) was supplied by China Resources in Liaoning Benxi
Third Pharmaceutical Co., Ltd. Celecoxib Capsules (Cele) (NO: DK1011)
were purchased from Pfizer. Carrageenan (NO: C804872) was purchased
from Shanghai Macklin Biochemical Co., Ltd. Isoflurane (NO: R510-22)
was purchased from RWD Life Science Co., Ltd. RIPA lysis buffer (NO:
P0013B), phosphatase inhibitor cocktail (NO: P1081), and phenylmethylsulfonyl
fluoride (PMSF) (NO: ST506) were purchased from Beyotime Biotechnology.
A Pierce Rapid Gold BCA Protein Assay Kit (NO: A53227) was purchased
from Thermo Scientific. SAPK/JNK Antibody (NO: 9252), Phospho-SAPK/JNK
Antibody (NO: 9251), and p38 MAPK Antibody (NO: 8690) were purchased
from Cell Signaling Technology. p-p38 Antibody (NO: WLP1576), ERK1/2
Antibody (NO: WL02371), p-ERK1/2 Antibody (NO: WLP1512), NF-κB
p65 Antibody (NO: WL01273b), p-NF-κB p65 Antibody (NO: WL02169),
and COX-2 Antibody (NO: WL01750) were purchased from Wanleibio Co.,
Ltd. β-actin Antibody (NO: AC026) and HRP Goat Anti-Rabbit IgG
(H + L) (NO: AS014) were purchased from ABclonal. An Ultra-High Sensitivity
ECL Kit (NO: BL523A) was purchased from Biosharp. Mouse TNF-α,
IL-1β, and IL-6 ELISA kits were purchased from R&D.
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