Cell lysates were prepared by using a mammalian cell lysis kit (Sigma, USA). The protein expression by western blotting was repeated thrice for each cell line. The following primary antibodies; polyclonal VEGFA, bFGF, TGFβ1 and E-cadherin (Abcam, UK), beta Actin (Bioss, USA) and EGFR (Santa Cruz, USA) were used. Following the peroxidase-conjugated secondary IgG (H + L) (Jackson Immuno Research, USA), immune complexes were visualized using luminol (Bio-Rad, USA). The chemiluminescence from the specific bands was visualized by Molecular Imager GelDoc XR+ ChemiDoc XRS+ Imaging System (BioRad, USA). The expression ratios of VEGFA, E-cadherin, TGFβ1, EGFR, bFGF, and β-actin from the blots were analyzed using Molecular Imager GelDoc XR+ ChemiDoc XRS+ Imaging System and Image Lab Software 6.0.1 (Bio-Rad, USA). After determining the intensities of the obtained bands, normalized protein fold ratios were calculated by proportioning the intensity value of each band to the corresponding β-actin band for each dose level.
Basic fibroblast growth factor (bfgf)
Manufactured by Bio-Rad
Sourced in United States
BFGF is a recombinant human basic fibroblast growth factor (bFGF) product offered by Bio-Rad. It is a protein that stimulates the growth and proliferation of various cell types, including fibroblasts, endothelial cells, and smooth muscle cells.
Automatically generated - may contain errors
Lab products found in correlation
Image lab software 6, by Bio-Rad (1 mentions)
Molecular imager geldoc xr chemidoc xrs imaging system, by Bio-Rad (1 mentions)
Beta actin, by Bioss Antibodies (1 mentions)
E cadherin, by Abcam (1 mentions)
Tgf β1, by Abcam (1 mentions)
Basic fibroblast growth factor (bfgf), by Abcam (1 mentions)
Luminol, by Bio-Rad (1 mentions)
β actin, by Bio-Rad (1 mentions)
Mammalian cell lysis kit, by Merck Group (1 mentions)
Tgfβ1, by Bio-Rad (1 mentions)
E cadherin, by Bio-Rad (1 mentions)
Mkn 45, by National Collection of Authenticated Cell Cultures (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Tnf α, by Bio-Rad (1 mentions)
Ifn γ, by Bio-Rad (1 mentions)
Mip 1β, by Bio-Rad (1 mentions)
Gm csf, by Bio-Rad (1 mentions)
2 protocols using basic fibroblast growth factor (bfgf)
1
Quantitative Protein Expression Analysis
2
Anticancer Peptide Effects on Gastric Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The anticancer active peptide used in the present study, anticancer bioactive peptide (ACBP) is a low-molecular-weight active substance that was obtained from the Clinical Medical Research Center, Affiliated Hospital of Inner Mongolia Medical University (Hohhot, China). The poorly differentiated human gastric adenocarcinoma cell line of MKN-45, the highly differentiated human gastric adenocarcinoma cell line NCI-N87 and the immortalized and non-tumorigenic human gastric mucosal epithelial cell line GES-1 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). RPMI-1640 medium and high-glucose DMEM were purchased from Hyclone (Cytiva). Penicillin, streptomycin, fetal bovine serum and 0.25% trypsin were from Gibco (Thermo Fisher Scientific, Inc.).
The Bio-Plex ProT Human Cytokine 27-plex Assay (cat. no. M500KCAFOY; Bio-Rad Laboratories, Inc.) is a multiplex assay that detects cytokines including IL-1β, IL-1RA, IL-2, IL-4, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17, Eotaxin, bFGF, GM-CSF, IFN-γ, MCP-1 (MCAF), IP-10, MIP-1α, PDGF-BB, MIP-1β, RANTES, TNF-α and VEGF. OXA was obtained from Jiangsu Hengrui Pharmaceutical Co., Ltd.
The Bio-Plex ProT Human Cytokine 27-plex Assay (cat. no. M500KCAFOY; Bio-Rad Laboratories, Inc.) is a multiplex assay that detects cytokines including IL-1β, IL-1RA, IL-2, IL-4, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12, IL-13, IL-15, IL-17, Eotaxin, bFGF, GM-CSF, IFN-γ, MCP-1 (MCAF), IP-10, MIP-1α, PDGF-BB, MIP-1β, RANTES, TNF-α and VEGF. OXA was obtained from Jiangsu Hengrui Pharmaceutical Co., Ltd.
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