Cells were harvested using 0.25% trypsin-EDTA. The solution was then centrifuged and cells were resuspended in a serum-free media containing 0.1% BSA. Six hundred microliters of complete medium containing 10% FBS was then added to the lower layer of Transwell 24-well plates (Millipore, Burlington, MA, USA). Cell suspension in the upper layer (200 µL, 4×105 cells) was incubated at 37°C and 5% CO2. Migration and invasion were assessed after 24 and 50 hours, respectively, by fixing the cells with methanol for 30 minutes and staining the cells with 0.1% crystal violet for 15 minutes.
Transwell 24 well plates
Manufactured by Merck Group
Sourced in United States
Transwell 24-well plates are a laboratory tool used for cell culture applications. The plates consist of a upper and lower chamber separated by a porous membrane, allowing for the study of cell migration, permeability, and other cell-based assays. The core function of the Transwell 24-well plates is to facilitate the co-culture of different cell types and the analysis of their interactions.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using transwell 24 well plates
1
Cell Migration and Invasion Assay
2
Cell Migration and Invasion Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Migration (without Matrigel) and invasion (with Matrigel) assays were conducted using transwell 24-well plates (8-μm pores; Millipore). A total of 5 × 10 4 cells were suspended in the upper chamber in 150 μL serum-free medium, and 600 μL normal growth medium was placed in the lower chamber. Cells which migrated/invaded through to the under-side of the membrane were xed, stained, and counted in 5 random elds of view.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!