6890n gc fid system

GC was carried out using a Agilent 6890 N GC-FID system, equipped with a flame ionization detector (FID) on a Agilent capillary column, HP-5 (30 m × 0.32 mm; film thickness 0.25 μm). The column temperature was programed from 40 °C to 250 °C for 5 °C/min. The column temperatures of injector and detector were fixed to 250 °C. Helium was used as the carrier, flow rate 1.0 ml/min.
The GC–MS analysis was carried out using a Agilent 6890 N GC-FID system, equipped with a flame ionization detector (FID) on a Agilent capillary column, HP-5 (30 m × 0.32 mm; film thickness 0.25 μm). The column temperature was programed from 40 °C to 250 °C at a rate of 5 °C/min. The column temperatures of injector and detector were 250 °C. Helium was used as the carrier, flow rate 1.0 ml/min. Split ratio was 1:100. The GC–MS analysis was done in the EI mode at 70 eV, inlet temperature was 200 °C and transfer line temperature was 250 °C. The temperature program was the same with that of the GC analysis. The injected volume was 0.2 μl.

The gas chromatography (GC) analyses were carried out using an 6890N GC-FID system equipped with DB-5 capillary column (30 m × 0.25 mm; film thickness of 0.25 mm) (Agilent Technologies, Santa Clara, United States) connected to an FID detector. The injector and detector temperatures were set at 280 °C at a rate of 5 °C/min. The carrier gas was helium (>99.2% purity) at a flow rate of 1.3 mL/min. All samples were analyzed in duplicate. Relative component concentrations were calculated based on GC peak areas without using correction factors [18] .