Human osteopontin quantikine kit

Manufactured by R&D Systems

Sourced in United States

The Human Osteopontin Quantikine kit is a quantitative sandwich enzyme immunoassay designed to measure human osteopontin levels in cell culture supernates, serum, and plasma. The kit provides necessary reagents and instructions to perform the assay.

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Close spelling variants of this product

Quantikine kits (80 citations) Osteopontin (26 citations) Human Osteopontin Quantikine ELISA Kit (6 citations) Quantikines (4 citations) Quantikine Human (2 citations) Human kits (2 citations)

2 protocols using human osteopontin quantikine kit

Replication of OPN Loci in YFS Cohort

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The three OPN loci identified in the GWAS of GCKD participants were tested for replication in the Cardiovascular Risk in Young Finns Study (YFS) cohort. Here, plasma OPN was measured by enzyme-linked immunosorbent assay (Human Osteopontin Quantikine kit, R&D Systems, USA) from samples thawed for the first time for the assay in 2007. Samples of 2,442 participants and 546,677 genotyped SNPs were available for further analysis after QC and imputation. Further details can be found in S1 Methods.
Per selected locus, association analysis of log₂(OPN) on SNP dosage (additive) was performed by fitting linear regression models adjusted for age, sex, and eGFR by using SNPTEST v2.5.4 [89 (link)]. GFR was estimated with the MDRD study equation and log₂-transformed prior to analysis [108 (link)]. Replication was defined by a one-sided association p-value <0.05/3 (Bonferroni correction for three OPN loci).

Cheng Y., Li Y., Scherer N., Grundner-Culemann F., Lehtimäki T., Mishra B.H., Raitakari O.T., Nauck M., Eckardt K.U., Sekula P, & Schultheiss U.T. (2022). Genetics of osteopontin in patients with chronic kidney disease: The German Chronic Kidney Disease study. PLoS Genetics, 18(4), e1010139.

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Galectin-3 and Osteopontin Levels in Centenarians

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Serum galectin-3 (Biovendor, Modrice, Czech Republic) and osteopontin levels were determined using commercially available enzyme-linked immunosorbent assay kits according to the manufacturers' protocols (Human Osteopontin Quantikine kit, R&D Systems, Minneapolis, MN, USA). Details for each assay are available from the authors upon request. For all assays, the intra-and inter-assay coefficients of variation were < 7% and < 9%, respectively. Each sample was analyzed in duplicate, and the mean value of the two measures was used for the analyses. Laboratory personnel were blinded with regard to subjects' group (centenarians or controls).

Sanchis-Gomar F., Santos-Lozano A., Pareja-Galeano H., Garatachea N., Alis R., Fiuza-Luces C., Morán M., Emanuele E, & Lucia A. (2016). Galectin-3, osteopontin and successful aging. Clinical chemistry and laboratory medicine, 54(5).

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