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Poloxamer 407 pol 407

Manufactured by Merck Group
Sourced in India, United States

Poloxamer-407 (Pol-407) is a non-ionic, water-soluble, thermoreversible gel-forming agent. It is a triblock copolymer composed of a central hydrophobic chain of polypropylene oxide (PPO) flanked by two hydrophilic chains of polyethylene oxide (PEO). Poloxamer-407 is commonly used as an excipient in pharmaceutical and biotechnology applications.

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2 protocols using poloxamer 407 pol 407

1

Lipid-based DOX Delivery System

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DOX was collected from Amneal Pharmaceuticals Ltd (Ahmedabad, India) as a gift sample. Apifil, Compritol 888 ATO, Compritol HD5 ATO, Capryol 90, and Precirol ATO 5 (PA) were collected as gift samples from Gattefosse Pvt. Ltd. (Mumbai, India). Stearic acid, Tween 80, and Poloxamer-407 (Pol-407) were procured from Sigma-Aldrich (Bangalore, India). Hydrogenated soybean phosphatidylcholine (HSPC) 50 was collected from the Sun Pharma Advanced Research Centre (SPARC, Vadodara) as a gift sample.
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2

Asparaginase Enzymatic Assay Protocol

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Asparaginase was obtained from Changzhou Qianhong Bio-Pharma Co., Ltd. (Jiangsu, China). Asparagine, aspartic acid, and Poloxamer 407 (Pol 407 ) were obtained from Sigma (USA). BSA, Dulbecco's modified Eagle's medium (DMEM), glutamine, tetrazolium bromide (MTT), and Nessler reagent were obtained from Sigma-Aldrich (USA). Dimethyl sulfoxide (DMSO) was obtained from Fisher Scientific (USA) and fetal bovine serum (FBS) from Biochrom (Berlin, Germany).
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