Qev original sec column sp5

STX-S and STX-N cells were cultured in FreeStyle medium (catalog number 12338018; Thermo Fisher) in a Multitron incubator (Infors HT). Subsequently, cells and cell debris were removed by centrifugation, while microvesicles and other extracellular vesicles larger than ~220 nm were removed by vacuum filtration. Next, exosomes were isolated using either Capricor’s laboratory-scale or large-scale purification method. For the laboratory-scale method, the supernatant was subjected to concentrating filtration using a Centricon Plus-70 centrifugal filter unit (catalog number UFC710008; Millipore) and then subjected to size exclusion chromatography (SEC) using a qEV original SEC column (SP5; Izon). For the large-scale method, the supernatant was subjected to concentrating tangential flow filtration (TFF) on an Äkta Flux s instrument (Cytiva, USA) and then subjected to size exclusion chromatography on an Äkta Avant 25 system (Cytiva, USA).

STX-S and STX-N cells were cultured in FreeStyle medium (catalog number 12338018; Thermo Fisher) in a Multitron incubator (Infors HT). Subsequently, cells and cell debris were removed by centrifugation, while microvesicles and other extracellular vesicles larger than ~220 nm were removed by vacuum filtration. Next, exosomes were isolated using either Capricor’s laboratory-scale or large-scale purification method. For the laboratory-scale method, the supernatant was subjected to concentrating filtration using a Centricon Plus-70 centrifugal filter unit (catalog number UFC710008; Millipore) and then subjected to size exclusion chromatography (SEC) using a qEV original SEC column (SP5; Izon). For the large-scale method, the supernatant was subjected to concentrating tangential flow filtration (TFF) on an Äkta Flux s instrument (Cytiva, USA) and then subjected to size exclusion chromatography on an Äkta Avant 25 system (Cytiva, USA).