Human breast cancer cell lines were purchased from the ATCC. Primary culture of normal human mammary gland duct epithelium (HMEC) was purchased from LONZA (Switzerland). Immortalized normal human mammary gland duct epithelium (HMEC4htertshp16) was kindly gifted from Dr. Tohru Kiyono (the National Cancer Center Research Institute, Japan) and Dr. Denis Galloway (Fred Hutchinson Cancer Research Center).
Human breast cancer cell lines
Manufactured by American Type Culture Collection
Sourced in United States
The human breast cancer cell lines are a collection of immortalized cell lines derived from human breast cancer tissue samples. These cell lines provide a standardized and well-characterized model for the study of breast cancer biology, including research on cancer cell growth, signaling pathways, and drug testing.
Automatically generated - may contain errors
Lab products found in correlation
Dharmafect 4, by Thermo Fisher Scientific (2 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions)
Mycoalert kit, by Lonza (2 mentions)
Human mammary gland epithelia cell line, by American Type Culture Collection (2 mentions)
Heat inactivated fetal bovine serum, by Thermo Fisher Scientific (1 mentions)
Megm complete medium, by Lonza (1 mentions)
Mcf 10a, by Lonza (1 mentions)
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Mycoalert mycoplasma detection kit, by Lonza (1 mentions)
Dulbecco modified eagle medium f12, by Merck Group (1 mentions)
Mcf 10a, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
13 protocols using human breast cancer cell lines
1
Culturing Breast Cancer and Normal Mammary Cells
2
Breast Cancer Cell Line Authentication
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Human breast cancer cell lines were purchased from ATCC (Manassas, VA) and most cell experiments were performed within 6 months after their receipt. However, after the completion of experiments outlined in this paper, the identity of all cell lines used in this study was confirmed by the Human Cell Line Authentication test (Genetica DNA Laboratories, Burlington, NC). Cells were cultured in a base medium supplemented with 10% heat-inactivated fetal bovine serum (Life Technologies), 50 units/mL penicillin, and 50 μg/mL streptomycin. The base medium for MDA-MB-231 was DMEM, for HCC1954 was RPMI, and for MCF-7 cells was insulin-supplemented MEM (all from Fisher Scientific, Pittsburgh, PA). MCF-10A cells were grown in MEGM complete medium (Lonza, Basel, Switzerland) that was replaced by RPMI containing 10% FBS 24 hours prior to treatment with blood samples. Serum, plasma, purified IgG or mAbs were used to treat cells. MAbs used in our studies include the LeY reactive MAb BR55–2 (a gift from Zenon Steplewski [74 (link)]), 14G2a (BD Biosciences, San Jose, CA) and 3F8 (a gift from Dr. Ni-Kong V. Cheung, Memorial Sloan Kettering Cancer Center, New York, NY).
3
Human Breast Cancer Samples and Cell Lines
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Samples from breast cancer patients (tumour tissues and adjacent normal tissues) were obtained from the Affiliated Hospital of Jiangnan University; these samples were composed of 96 human primary breast cancer tissues and 64 paired adjacent noncancerous tissues. This project was authorized by the Research Ethics Committees of the corresponding institutions, and informed consent was obtained from all patients. In total, four human breast cancer cell lines were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA). The cell lines were characterized using short tandem repeat (STR) markers by the Genetic Testing Biotechnology Corporation (Suzhou, China). MDA-MB-231, ZR-75-30, and Hs578T cells were cultured in RPMI DMEM. BT-549 cells were cultured in RPMI 1640. These two complete media both included 10% foetal bovine serum and 1% streptomycin and penicillin. All materials were obtained from the same company (HyClone, Utah, USA). All cells were cultured at 37°C with 5% (v/v) CO2 in a humidified chamber.
4
Cell Line Authentication and Mycoplasma Testing
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Human breast cancer cell lines were obtained from American Type Culture Collection (ATCC, Teddington, UK). All cells were routinely cultured in RPMI 1640 (Life Technologies 11965), or Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% FBS recommended by suppliers. The cell lines were authenticated by short-tandem-repeat (STR) analysis and matched to the German Collection of Microorganisms and Cell Cultures (DSMZ) database, and they were used for no more than 25 passages after STR typing. Mycoplasma tests were routinely performed using MycoAlert Mycoplasma Detection Kit (Lonza, Basel, Switzerland).
5
Conditioned Media Collection from Breast Cancer Cells
6
Culturing Human Breast Cell Lines
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Human breast cancer cell lines were obtained from the American Type Culture Collection (Manassas, VA). All cell lines were cultured in Dulbecco modified Eagle medium/F12 supplemented with 10% fetal bovine serum (Sigma-Aldrich, St. Louis, MO), except for non-tumorigenic human breast cells (MCF10A from ATCC), which were cultured in Dulbecco modified Eagle medium/F12 supplemented with 5% horse serum, epidermal growth factor, hydrocortisone, insulin, and cholera toxin (Calbiochem). Cells were cultured at 37°C in a humidified incubator with 5% CO2.
7
Standardized Cell Line Culture
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Human breast cancer cell lines were purchased from the American Type Culture Collection. All cell lines were cultured following standard guidelines. All cell lines were maintained without antibiotics in an atmosphere of 5% CO2 and 99% relative humidity at 37°C. Cell lines were passaged for fewer than 6 months and were authenticated by short tandem repeat analysis. No mycoplasma infection was found for all cell lines.
8
Isolation and Culture of Mouse Embryonic Fibroblasts
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Human breast cancer cell lines were obtained from American Type Culture Collection (ATCC, USA). SKBR3 and BT474 cells were cultured in DMEM/F12 (GIBCO/Invitrogen, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS). All cells were maintained at 37°C in an incubator containing 5% CO2. Primary mouse embryonic fibroblasts (MEFs) were derived from Ptpro+/+ and Ptpro−/− mice. In brief, uteri firstly isolated from 13.5-days-pregnant mice, then washed uteri with phosphate-buffered saline (PBS) and removed the head and visceral tissues. After washed with fresh PBS, the remaining bodies were minced using a pair of scissors, transferred into a 0.25% trypsin/EDTA solution (1 mL per embryo) at 37°C for 20 min. The trypsin was inactivated by DMEM with 10% FBS. The cells were cultured for 24 h at 37°C. Adherent cells were used as MEF cells.
9
Cell Line Maintenance for Breast Cancer
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Human breast cancer cell lines were purchased from the American Type Culture Collection. All cell lines were cultured following standard guidelines. All cell lines were maintained without antibiotics in an atmosphere of 5% CO2 and 99% relative humidity at 37°C. Cell lines were passaged for fewer than 6 months and were authenticated by short tandem repeat analysis. No mycoplasma infection was found for all cell lines.
10
Characterization of Human Cell Lines
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