Human chorionic gonadotropin (hcg)

Manufactured by MSD animal health

Sourced in Netherlands, Belgium, Switzerland

HCG is a laboratory equipment product manufactured by MSD animal health. It is designed to measure the levels of human chorionic gonadotropin (HCG), a hormone produced during pregnancy. The core function of HCG is to provide accurate and reliable measurements of HCG levels in biological samples.

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Lab products found in correlation

Human chorionic gonadotropin (hcg), by MSD (4 mentions) Chorulon, by MSD animal health (1 mentions) Alexa fluor 633, by Thermo Fisher Scientific (1 mentions) Mouse monoclonal antibodies, by Santa Cruz Biotechnology (1 mentions) Bovine trypsin, by Roche (1 mentions) M8410, by Merck Group (1 mentions) Mr 107 d, by Merck Group (1 mentions) A5177 5ea, by Merck Group (1 mentions) A3311, by Merck Group (1 mentions) G1 medium, by Vitrolife (1 mentions)

4 protocols using human chorionic gonadotropin (hcg)

Ovarian Cycle Staging in Mice

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The oestrous cycle was monitored studying vaginal cytology. Cells collected via saline lavage were placed on glass slide and stained with Diff Quik® kit (Medion Diagnostics AG, Switzerland, DQ-ST). Oestrus was characterised by cornified epithelium cells; metoestrus by both cornified cells and leukocytes; dioestrus by predominant leukocytes; and pro-oestrus by nucleated cells, as previously described (Kyrönlahti et al. 2011) (link).
Group of female B6 mice (8 wk old) was injected in oestrus stage with eCG (G4877, 5IU, Sigma Aldrich, Saint Louis, Missouri, USA) followed after 48 h by hCG (Chorulon, 5IU, MSD Animal Health, Boxmeer, Netherlands) and tissues collected 18-20 h later in E. The second group of mice were injected with hCG and tissues were collected 16-18 h later in D. To reduce variation between groups ovaries from females from the remaining experiments were collected in dioestrus stage.

Adamowski M., Wołodko K., Oliveira J., Castillo‐Fernandez J., Murta D., Kelsey G., & Galvão A. (2021). Leptin Signalling in the Ovary of Diet-Induced Obese Mice Regulates Activation of Nod-Like Receptor Protein 3 Inflammasome.

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Immunofluorescence Analysis of Oviductal Proteins

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All reagents were purchased from Sigma-Merck (Saint-Louis, MO, USA) if not otherwise stated. PMSG, hCG and PG-600 were obtained from MSD Animal Health (Brussels, Belgium). Bovine trypsin was obtained from Roche Diagnostics GmbH (Basel, Switzerland). Paraformaldehyde (sc-281692) and mouse monoclonal antibodies raised against OVGP1 (sc-377267) and PYGL (sc-517597) were obtained from Santa-Cruz Biotechnology (Dallas, TX, USA). Goat polyclonal antibody raised against ANXA1 (AP22515PU-N) was obtained from Origene (Rockville, MD, USA). Secondary antibodies coupled with Alexa Fluor 633 (A21050 for ANXA1; A21082 for OVGP1 and PYGL) were obtained from Invitrogen Molecular Probes (Eugene, OR, USA).

Banliat C., Tsikis G., Labas V., Teixeira-Gomes A.P., Com E., Lavigne R., Pineau C., Guyonnet B., Mermillod P, & Saint-Dizier M. (2020). Identification of 56 Proteins Involved in Embryo–Maternal Interactions in the Bovine Oviduct. International Journal of Molecular Sciences, 21(2), 466.

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Embryo Isolation and Culture for Myosin-X Knockout Mice

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Embryos were isolated from superovulated female mice (Myo10^flox/flox; Cre⁺ and Myo10^flox/flox; Cre⁻ as Myo10^−/− full mice, and Myo10^wt/wt; Cre⁺ and Myo10^wt/wt; Cre⁻ as control mice) mated with male mice (Myo10^wt/wt; Cre⁻). Superovulation of female mice was induced by intraperitoneal injection of 5 IU pregnant mare’s serum gonadotropin (Ceva, Syncro-part), followed by intraperitoneal injection of 5 IU human chorionic gonadotropin (MSD Animal Health, Chorulon) 44–48 h later. Embryos were recovered at E0.5 from plugged females by opening the ampulla followed by a brief treatment with 37°C 0.3 mg/ml hyaluronidase (H4272-30MG; Sigma-Aldrich) and washing in 37°C FHM. When ampulla was not present, embryos were recovered by flushing oviducts with 37°C FHM (MR-122-D; Millipore) using a modified syringe (1400 LL 23; Acufirm). Embryos were handled using an aspirator tube (A5177-5EA; Sigma-Aldrich) equipped with a glass pipette pulled from glass micropipettes (Blaubrand intraMark or Warner Instruments). Embryos were placed in KSOM (MR-107-D; Millipore) supplemented with 0.1% BSA (A3311; Sigma-Aldrich) in 10 ml droplets covered in mineral oil (M8410; Sigma-Aldrich). Embryos were cultured in an incubator under a humidified atmosphere supplemented with 5% CO₂ at 37°C for 5 d. Embryos were scored for survival and embryonic stage from E0.5 to E4.5.

Crozet F., Letort G., Bulteau R., Da Silva C., Eichmuller A., Tortorelli A.F., Blévinal J., Belle M., Dumont J., Piolot T., Dauphin A., Coulpier F., Chédotal A., Maître J.L., Verlhac M.H., Clarke H.J, & Terret M.E. (2023). Filopodia-like protrusions of adjacent somatic cells shape the developmental potential of oocytes. Life Science Alliance, 6(6), e202301963.

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Mouse Oocyte Isolation and Embryo Culture

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These procedures were performed as described previously [8 (link),16 (link),30 (link)]. Briefly, 8- to 12-week-old female FVB mice were superovulated by IP injection of 5 IU (0.1 mL) pregnant mare serum gonadotropin (Intervet, Zürich, Switzerland), followed 50 h later by an IP injection of 5 IU (0.1 mL) of human chorionic gonadotropin (hCG; MSD Animal Health, Luzern, Switzerland). Fourteen hours after hCG injection, cumulus–oocyte complexes were recovered from oviducts in HTF supplemented with 5 mg/mL of HSA. Spermatozoa were collected from the cauda epididymis of 10- to 14-week-old FVB mice and capacitated for 60 min in HTF/HSA medium at 37 °C under a humidified atmosphere of 6% CO₂ in air. Oocytes were inseminated 14 h after hCG with 10 × 10⁻⁶ spermatozoa in HTF/HSA medium for 4 h at 37 °C and 6% CO₂. Eggs were then transferred to 25-µL drops of G1 medium (Vitrolife, Göteborg, Sweden) covered with paraffin oil. The embryo culture was conducted up to the blastocyst stage in sequential G1 and G2 (Vitrolife, Göteborg, Sweden) medium pre-equilibrated at 37 °C under the CO₂-enriched atmosphere. Embryos were kept in the G2 medium for 48 h before the transfer to pseudopregnant females. The culture media used in these studies were largely used during ART in humans.

Meister T.A., Soria R., Dogar A., Messerli F.H., Paoloni-Giacobino A., Stenz L., Scherrer U., Sartori C, & Rexhaj E. (2022). Increased Arterial Responsiveness to Angiotensin II in Mice Conceived by Assisted Reproductive Technologies. International Journal of Molecular Sciences, 23(21), 13357.

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