Bcl2 associated x (bax)

FeCl₂·4H₂O, FeCl₃·6H₂O, diethyleneglycol (DEG), Zn(NO₃)₂·6H₂O, and NaOH were purchased from Shanghai Chemical Reagents Co (Shanghai, China). Dox was acquired from Hisun Phamaceuticals, Zhejiang, China. Cell Counting Kit-8 (CCK-8) and 2′,7′-dichlorofluorescin diacetate (DCFH-DA) were obtained from Sigma (St Louis, MO). RPMI-1640 culture medium, fetal bovine serum (FBS), and cultured plate were purchased from Gibco (Gibco/BRL, Carlsbad, CA). The antibodies of Bax, Bcl-2, Casepase 3, β-actin and horseradish peroxidase-conjugated IgG antibody were obtained from Nanjing KeyGen Biotech. Inc (Nanjing, China). All other reagents were of analytical grades.

Ringer's solution, containing 122 mM sodium chloride, 3 mM potassium chloride, 0.4 mM monopotassium phosphate, 1.2 mM magnesium sulfate, 25 mM sodium bicarbonate, and 1.2 mM calcium chloride, was purchased from Millipore (MA, USA). Pachymic acid, liquiritin, rhynchophylline, isorhynchophylline, corynoxeine, and isocorynoxeine (purity ≥ 99%) were purchased from National Institutes for Food and Drug Control (Beijing, China). Fetal bovine serum (FBS) was purchased from Gibco/BRL (Grand Island, NY, USA). Methyl thiazolyl tetrazolium (MTT), dimethyl sulfoxide (DMSO), and trypsin were provided by Sigma Chemical (St. Louis, MO, USA). Aβ_1–42 (purity ≥ 95%) was purchased from Abcam (Cambridge, USA). HPLC-grade acetonitrile was purchased from Thermo Fisher Scientific (Massachusetts, USA). EliVision plus and DAB kits were provided by Fuzhou Maixin Biotech. Co., Ltd. (Fuzhou, China). Bax, Bcl-2, caspase-3 and caspase-9 antibodies, annexin V-FITC/PI assay kit, basal DMEM medium, cell lysis solution, and 0.1% DEPC water were purchased from Nanjing KeyGen Biotech Co., Ltd. (Nanjing, China). Nimodipine was provided by Bayer healthcare Co., Ltd. (Leverkusen, Germany).