HUVEC pools were purchased from Invitrogen (Breda, The Netherlands). EGM-2 medium and SingleQuots™ for HUVEC cell culture were purchased from Lonza (Verviers, Belgium). PAI-1 inhibitors PAI-039 (tiplaxtinin) and TM5275 were purchased from Axon Medchem BV (Groningen, The Netherlands) and diluted in DMSO (referred to as solvent). PAI-1 rabbit polyclonal antibody was a kind gift from Dr. Sacha Zeerleder. VE-cadherin monoclonal mouse antibody, clone BV6, was purchased from Millipore (Amsterdam, The Netherlands) and actin [AC-40] monoclonal mouse antibody was purchased from Sigma (Zwijndrecht, The Netherlands). GM130 rabbit monoclonal antibody was from Cell Signaling (Leiden, The Netherlands). Goat polyclonal anti-VE-cadherin [C-19] and rabbit polyclonal anti-α-catenin were purchased from Santa Cruz Biotechnology (Heidelberg, Germany). Mouse monoclonal antibodies directed against mouse monoclonal PECAM-1 AF488 [WM59], VE-cadherin AF 647 [55-7H1], IgG1 AF674, β-catenin [14 (link)] and p120 catenin [98/pp120] were purchased from BD Biosciences (Amsterdam, The Netherlands). Texas Red-X Phalloidin, chicken anti-goat AF647, chicken anti-rabbit AF488, chicken anti-rabbit AF594, chicken anti-mouse AF488 and IgG1 AF488 were from Invitrogen (Breda, The Netherlands).
Tm5275
Manufactured by Axon Medchem
Sourced in United States
The TM5275 is a laboratory equipment that performs temperature measurement and control functions. It features a digital display and user-friendly controls for setting and monitoring temperature parameters. The device is designed for use in various scientific and research applications that require precise temperature regulation.
Automatically generated - may contain errors
Lab products found in correlation
Chicken anti goat af647, by Thermo Fisher Scientific (1 mentions)
Texas red x phalloidin, by Thermo Fisher Scientific (1 mentions)
Anti α catenin, by Santa Cruz Biotechnology (1 mentions)
Chicken anti rabbit af594, by Thermo Fisher Scientific (1 mentions)
Chicken anti mouse af488, by Thermo Fisher Scientific (1 mentions)
Chicken anti rabbit af488, by Thermo Fisher Scientific (1 mentions)
Egm 2 medium, by Lonza (1 mentions)
Fibronectin solution, by Fujifilm (1 mentions)
Trypsin edta, by Fujifilm (1 mentions)
Dulbecco modified eagle medium (dmem), by Fujifilm (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Caski, by American Type Culture Collection (1 mentions)
2 protocols using tm5275
1
HUVEC Cell Culture and Protein Analysis
2
Cervical Cancer Cell Culture and Extracellular Matrix Coating
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell culture. The cervical cancer cell lines CaSki and SiHa were obtained from American Type Culture Collection (ATCC, USA) and cultured in Dulbecco's modified Eagle's medium (DMEM; Wako, Japan) supplemented with 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA) and sub-cultured by 0.25% trypsin/EDTA (Wako) detachment. The cells were grown in a humidified atmosphere at 37˚C and 5% CO 2 .
Coatings. Fibronectin solution, vitronectin solution and laminin solution were purchased from Wako (Cat# 063-05591, 220-02041 and 120-05751, respectively), and were used according to manufacturer's instructions. Collagen I and collagen IV-coated plates were obtained from Corning (Acton, MA, USA).
Reagents. Human PAI-1 peptide was purchased from Abcam (Cambridge, MA, USA). TM5275 was purchased from Axon Medchem (USA).
Coatings. Fibronectin solution, vitronectin solution and laminin solution were purchased from Wako (Cat# 063-05591, 220-02041 and 120-05751, respectively), and were used according to manufacturer's instructions. Collagen I and collagen IV-coated plates were obtained from Corning (Acton, MA, USA).
Reagents. Human PAI-1 peptide was purchased from Abcam (Cambridge, MA, USA). TM5275 was purchased from Axon Medchem (USA).
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