K562 cells

Manufactured by National Collection of Authenticated Cell Cultures

Sourced in China

K562 cells are a continuous human myelogenous leukemia cell line derived from a 53-year-old female patient. They are suspension cells and can be used for various cell biology and molecular biology experiments.

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Lab products found in correlation

Fitc conjugated anti nkg2d, by Thermo Fisher Scientific (1 mentions) Fitc conjugated anti cd158a, by BD (1 mentions) Pe conjugated anti cd158b, by BD (1 mentions) Percp conjugated anti cd3, by BD (1 mentions) Apc conjugated anti nkp46, by BD (1 mentions) Pe cy7 conjugated anti cd56, by BD (1 mentions) Pe conjugated anti granzyme b, by BD (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions) Hepg2 cells, by National Collection of Authenticated Cell Cultures (1 mentions)

3 protocols using k562 cells

NK Cell Cytotoxicity Assay with K562 Cells

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K562 cells (China Center for Type Culture Collection, Wuhan, China) were used as target cells in the NK cytotoxicity assay. K562 cells were cultured in RPMI 1640 (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Hyclone, Logan, UT, USA). 3,3′-Dioctadecyloxacarbocyanine perchlorate (DIO) (1911717; Invitrogen, San Diego, CA, USA) was dissolved in dimethyl sulfoxide (Sigma, St. Louis, MO, USA) to a concentration of 3 mM. Propidium iodide (PI; MKCB0899V; Sigma) was used to detect apoptosis.
Flow cytometry to identify NK cells and subpopulations expressing certain markers and effector molecules was performed using the following fluorophore-conjugated monoclonal mouse anti-human antibodies: PerCP-conjugated anti-CD3 (347344; BD Pharmingen, San Jose, CA, USA), PE-cy7-conjugated anti-CD56 (557747; BD Pharmingen), PE-conjugated anti-NKp30 (12–3379; eBioscience ThermoFisher, Waltham, MA, USA), APC-conjugated anti-NKp46 (17–3359; BD Pharmingen), FITC-conjugated anti-NKG2D (11–5878; eBioscience), FITC-conjugated anti-CD158a (556062; BD Pharmingen), PE-conjugated anti-CD158b (559785; BD Pharmingen), PE-conjugated anti-granzyme B (561142; BD Pharmingen), Alexa Fluor 647-conjugated anti-perforin (563576; BD Pharmingen), and Alexa Fluor 488-conjugated anti-granulysin (558254; BD Pharmingen).

Zhang H., Huang C., Chen X., Li L., Liu S., Li Y., Zhang Y., Zeng Y, & Hu L. (2019). The number and cytotoxicity and the expression of cytotoxicity-related molecules in peripheral natural killer (NK) cells do not predict the repeated implantation failure (RIF) for the in vitro fertilization patients. Genes & Diseases, 7(2), 283-289.

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Culturing Mammalian Cell Lines

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CHO cells and K562 cells (China Center for Type Culture Collection, Wuhan, PR China) were cultured in RPMI1640 medium (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS, Invitrogen) and 100 U/ml ampicillin, 100mg/ml streptomycin. HepG2 cells (China Center for Type Culture Collection, Wuhan, PR China) were cultured in DMEM complete growth medium (Invitrogen) supplemented with 10% FBS and antibiotics. All cells were incubated at 37°C in a humidified atmosphere containing 5% CO₂.

He Q., Sun X., Chu C., Jiang Q., Zhu H., He Y., Yue T., Wang R., Lei P, & Shen G. (2015). Endocytosis of a Functionally Enhanced GFP-Tagged Transferrin Receptor in CHO Cells. PLoS ONE, 10(3), e0122452.

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K562 Cell Culture Protocol

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The K562 cells (China Center for Type Culture Collection, Wuhan, China) were grown in RPMI-1640 medium supplemented with 10% calf serum, 100 μg/ml streptomycin (North China Pharmaceutical Co., Ltd., Shijiazhuang, China) and 100 U/ml penicillin (North China Pharmaceutical Co., Ltd.) in a humidified 5% CO₂ and 95% air incubator at 37°C.

WANG Q., WANG Y.L., WANG K., YANG J.L, & CAO C.Y. (2014). Polyamine analog TBP inhibits proliferation of human K562 chronic myelogenous leukemia cells by induced apoptosis. Oncology Letters, 9(1), 278-282.

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