Cyclohexyl isocyanide

Allyl-alcohol (99%), benzyl alcohol (99%), butanol (99.5%), 1-octanol (99%), phenyl acetaldehyde (98%, stabilized) and trimethylamine (99.7%) were purchased from Acros Organics. 1-Adamantyl isocyanide (95%), cyclohexanol (99%), cyclohexyl isocyanide (98%), 2-butanol (99.5%, anhydrous), 2,4-dinitrobenzaldehyde (97%), isobutyraldehyde (≥99.5%), 2-morpholinoethyl isocyanide (≥98%), tert-butanol (≥99.5%, anhydrous), tert-butyl isocyanide (98%), tetradecane (≥99.5%) and undecylenic aldehyde (95%) were obtained from Sigma Aldrich. Other chemicals used include: diazabicyclo[5.4.0]undec-7-ene (DBU, TCl, >98%), deuterated chloroform (CDCI₃-d, Merck), dimethyl sulfoxide (DMSO, VWR, 99%) and trimethyl acetaldehyde (ABCR, 97%). Carbon dioxide (CO₂) with purity over 99.9% was obtained from Air Liquide. Cyclohexane, ethyl acetate and dichloromethane were distilled before usage, while tetrahydrofuran (THF) was of technical grade and used without further purification. All other chemicals were used as received from the supplier.

RPMI-1640 medium, lymphoprep^™, 0.9% saline (NaCl), millipore filter (0.22 μM), fetus calf serum (FCS), L-glutamine (200 mM; 100×), pen/strep (10,000 unit/ml pen and 10,000 units/ml strep), MEM sodium pyruvate (100 mM), non-essential amino acids (10 mM; 100×). IL-2 (100 IU/ml) were from GIBCO Life Technologies, (USA). Human recombinant interleukin-2 and interleukin-15 were from Novartis (Switzerland) and Miltenyi Biotec (Germany), respectively. Mouse monoclonal antibodies specific for CD3 (UCHT1), TCR-Vγ9 (Immu360) were from Beckman Coulter (USA). Fixable aqua dead cell stain kit was from Invitrogen-Life Technologies (USA). Human IgG, methanol, aniline, benzaldehyde, propiolic acid, cyclohexyl isocyanide, dimethyl sulfoxide (DMSO) and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltratrazolium bromide were purchased from Sigma Aldrich (USA). Zoledronic acid injection (4 mg) were purchased from Cipla, (India). HL-60 and K562 cell lines were received from ATCC (USA).