Postmortem brain lysates were prepared from slices of individual tissues, extracts were resolved by sodium dodecylsulfate-polyacrylamide gel electrophoresis followed by Western blotting as previously described [33] . The following antibodies were employed: a mouse monoclonal anti-LAMP2 (H4B4) (1:2000, Santa Cruz Biotechnology, sc18822), a mouse monoclonal anti-LAMP1 (H4A3) (1:2000, Santa Cruz Biotechnology, sc20011), a rabbit monoclonal anti-ACTIN (1:400, Sigma-Aldrich, A2066/030M4844), a monoclonal anti-α-Tubulin (clone DM1A, 1:10,000, Sigma-Aldrich T6199), a rabbit polyclonal Anti-Phospho-AKT473 antibody (1:500, Cell Signaling, 9271), a rabbit polyclonal Anti-AKT antibody (1:500, Cell Signaling, 9272), a rabbit polyclonal Anti-Phospho-ERK antibody (1:500, Cell Signaling, 4370) or a rabbit polyclonal anti-ERK antibody (1:500, Cell Signaling, 4695). The final detection was performed as previously described [33] .
Anti phospho akt473 antibody
Manufactured by Cell Signaling Technology
The Anti-Phospho-AKT473 antibody is a lab equipment product that detects the phosphorylation of the serine 473 residue on the AKT protein. It is used to measure the activation state of the AKT signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Anti erk antibody, by Cell Signaling Technology (2 mentions)
Anti phospho erk antibody, by Cell Signaling Technology (2 mentions)
Anti akt antibody, by Cell Signaling Technology (2 mentions)
Anti lamp1 h4a3, by Santa Cruz Biotechnology (2 mentions)
Anti α tubulin clone dm1a, by Merck Group (2 mentions)
Anti lamp2 h4b4, by Santa Cruz Biotechnology (2 mentions)
2 protocols using anti phospho akt473 antibody
1
Western Blot Analysis of Postmortem Brain Lysates
2
Postmortem Brain Protein Analysis
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!