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Arachidonyl trifluoromethylketone

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Arachidonyl trifluoromethylketone is a chemical compound used in laboratory research. It functions as a selective inhibitor of the enzyme cytosolic phospholipase A2.

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Lab products found in correlation

Cay10502, by Cayman Chemical (1 mentions) Aacocf3, by Merck Group (1 mentions) Syto9, by Thermo Fisher Scientific (1 mentions) Ionomycin, by Merck Group (1 mentions) Resorufin, by Merck Group (1 mentions) Resorufin sodium salt, by Merck Group (1 mentions) Bromoenol lactone, by Merck Group (1 mentions) Naphthofluorescein, by Merck Group (1 mentions) Palmitoyl chloride, by Merck Group (1 mentions) Arachidonoyl chloride, by Santa Cruz Biotechnology (1 mentions) Phospholipase a2 from porcine pancreas, by Merck Group (1 mentions) Ly311727, by Santa Cruz Biotechnology (1 mentions)

2 protocols using arachidonyl trifluoromethylketone

1

Quantifying Cell Injury and Survival in RPE Cells

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Lactate dehydrogenase (LDH) release was used to quantitatively access cell injury in cells exposed to SI. Inhibitors of PLA2 were used to identify their influence on RPE cell survival. The iPLA2-VIA-specific inhibitors included bromoenol lactone (BEL; BioNordika, Herlev, Denmark, Cayman Chemicals, Product number 13179) and 1, 1, 1, 2, 2- pentafluoro- 7- phenyl- 3- heptanone (FKGK; Cayman). Additionally, the cPLA2-IVA inhibitor CAY 10502 (Cayman) as well as the combined cPLA2-IVA and iPLA2-VIA inhibitor arachidonyl trifluoromethylketone (AACOCF3; Sigma) were used. LDH-release was assayed using a MWG Discovery HT-R spectrophotometer. In addition, cell viability was determined by the colorimetric assay 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) according to the manufacturer's instructions.
Kolko M., Vohra R., Westlund van der Burght B., Poulsen K, & Nissen M.H. (2014). Calcium-independent phospholipase A2, group VIA, is critical for RPE cell survival. Molecular Vision, 20, 511-521.
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2

Fluorometric Lipase Activity Assay

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Resorufin, Resorufin sodium salt, naphthofluorescein, palmitoyl chloride, phospholipase A2 from porcine pancreas, arachidonyl trifluoromethyl ketone, bromoenol lactone, ionomycin, and H2O2 30% (w/w) were purchased from Sigma-Aldrich. DDAO, arachidonoyl chloride, and LY311727 were purchased from Santa Cruz Biotechnology. 7-HCA was purchased from Cayman Chemical. Syto 9 was purchased from Life Technologies. Egg phosphatidylcholine was purchased from Avanti Polar Lipids, Inc.
Chiorazzo M.G., Bloch N.B., Popov A.V, & Delikatny E.J. (2015). Synthesis and Evaluation of Cytosolic Phospholipase A2 Activatable Fluorophores for Cancer Imaging. Bioconjugate chemistry, 26(12), 2360-2370.
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