Annexin 5 fitc 7 aad
Annexin V-FITC/7-AAD is a combination of fluorescent labels used to detect and quantify apoptosis in cells. Annexin V binds to phosphatidylserine, which is exposed on the cell surface during early stages of apoptosis. 7-AAD is a dye that penetrates cells with compromised cell membranes, indicating late-stage apoptosis or necrosis. This combination provides a reliable method to differentiate between viable, early apoptotic, and late apoptotic/necrotic cells.
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7 protocols using annexin 5 fitc 7 aad
Apoptosis Pathway Regulation Assay
Apoptosis Quantification via Flow Cytometry
Flow Cytometry-based Apoptosis Assay
Measuring Cell Apoptosis and TUNEL Assay
Terminal Deoxynucleotidyl Transferase (TdT)-Mediated dUTP Nick-End Labeling (TUNEL) assay was used to detect the apoptotic cells, according to the manufacturer’s instructions. Cochlear sections were permeabilized in 0.3% Triton X-100 for 60 min, and then blocked with a fluorometric terminal deoxytransferase solution for 1 h, at 37 °C. The sections were then incubated with anti-Tuj1 primary antibody overnight at 4 °C. They were then stained with a Cy3-conjugated secondary antibody for 2 h, at room temperature. Finally, the nuclei were stained with DAPI, and fluorescent images were taken using a Leica TCS SP5 confocal fluorescent microscope.
Evaluating NK Cell-Mediated Cytotoxicity
Apoptosis Analysis of KYSE-30 and TE-1 Cells
Siglec-8-Mediated Eosinophil Apoptosis
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