Cell light edu dna cell proliferation assay kit

The proliferation of cultured SCs was determined with a Cell-Light EdU DNA Cell Proliferation Assay Kit (Ribobio, Guangzhou, China). Transfected SCs were resuspended in complete medium, counted, and transferred to poly-L-lysine-coated 96-well plates. The SCs were fixed with formaldehyde in phosphate-buffered saline (PBS), observed under a microscope (Leica Microsystems, Wetzlar, Germany) and subjected to proliferation analysis.

Schwann cells were resuspended in fresh complete medium, counted and plated on poly-L-lysine-coated 96 well plates. EdU was applied and the cells were cultured after cell transfection for 48 hours. The cells were fixed with formaldehyde in PBS and assayed using a Cell-Light EdU DNA Cell Proliferation Assay Kit (Ribobio) following the manufacturer's protocol. Cell proliferation was expressed as the ratio of EdU-positive cells to total cells. Schwann cell proliferation analysis was performed using images of randomly selected fields obtained on a DMR fluorescence microscope (Leica Microsystems). All samples were analyzed in three independent experiments.