Universal abc detection kit

Manufactured by Vector Laboratories

The Universal ABC Detection Kit is a versatile tool designed for immunohistochemical and immunocytochemical staining applications. It provides a reliable and consistent method for detecting target antigens in various sample types. The kit contains a reagent system that amplifies the signal, enhancing the visualization of the target of interest.

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Lab products found in correlation

Dp72 microscope, by Olympus (5 mentions)

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ABC kit (558 citations) ABC detection kit (19 citations) Universal ABC kit (9 citations)

5 protocols using universal abc detection kit

Immunohistochemical Analysis of Tumor Tissues

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Tumor tissues from control, 2HF-treated, scrambled-antisense, and RLIP76-antisense mice were collected, fixed in buffered formalin for 24 h, embedded in paraffin, and 5-μm thick sections on poly-L-lysine–coated slides were prepared. Tissue sections were stained with hematoxylin and eosin staining (H&E), and RLIP76, Ki67, CD31, E-cadherin, and vimentin using Universal ABC detection kit (Vector). Immuno-reactivity is evident as a dark brown stain, whereas non-reactive areas are indicative of the background staining. Photomicrographs were acquired using Olympus DP 72 microscope with 40× magnifications.

Singhal J., Chikara S., Horne D., Salgia R., Awasthi S, & Singhal S.S. (2018). 2′-Hydroxyflavanone inhibits in vitro and in vivo growth of breast cancer cells by targeting RLIP76. Molecular carcinogenesis, 57(12), 1751-1762.

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Histopathological Analysis of Breast Tumors

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Breast tumors (control as well as 25, 50 and 100 mg/kg b.w. 2HF treated) were harvested from mice bearing tumors for 60 d. Tumor samples fixed in buffered formalin for 12 h were processed conventionally for paraffin-embedded tumor sections (5 μm thick). Hematoxylin and Eosin (H&E) staining to assess hyperplasia was performed on paraffin-embedded tumor sections. Histopathologic analyses for protein markers with anti-E cadherin to analyze tumor suppressor/ differentiation effects, anti-CD31 to visualize blood vessels, anti-Ki67 to assess cell proliferation, anti-vimentin to analyze mesenchymal cells, and anti-PCNA to assess proliferating cell nuclear antigen were also performed, using Universal ABC detection kit (Vector). Statistical significance of difference was determined by two-tailed Student’s t test. Immuno-reactivity is evident as a dark brown stain, whereas non-reactive areas display only the background color. Sections were counterstained with Hematoxylin (blue). Photomicrographs at 40x magnification were acquired using Olympus DP 72 microscope. Percent staining was determined by measuring positive immuno-reactivity per unit area. The intensity of antigen staining was quantified by digital image analysis using DP2-BSW software. Bars represent mean ± S.E. (n = 5); * p<0.001 compared with control.

Singhal J., Nagaprashantha L., Chikara S., Awasthi S., Horne D, & Singhal S.S. (2017). 2'-Hydroxyflavanone: A novel strategy for targeting breast cancer. Oncotarget, 8(43), 75025-75037.

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Immunohistochemical Analysis of EMT Markers

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Tumor tissues from control groups (corn oil, CAS, and PIS) and experimental groups (2HF, RAS, Rab, and 2HF+RAS+Rab) were fixed in buffered formalin for 12 h. Paraffin-embedded 5-μm thick tumor sections were prepared. Hematoxylin and eosin (H&E) staining to assess hyperplasia was performed on the paraffin-embedded tumor sections. Histopathologic analysis of proteins, such as E-cadherin and vimentin involved in epithelial-mesenchymal transition (EMT), CD31 to visualize blood vessels, Ki67 and RLIP to assess cell proliferation, and vimentin to analyze mesenchymal cells was performed using a Universal ABC detection kit (Vector). Immuno-reactivity is evidenced by a dark brown stain, whereas non-reactive areas display only the background color. Photomicrographs at 40x magnification were acquired using an Olympus DP72 microscope. Percent staining was determined by measuring positive immuno-reactivity per unit area. The intensity of antigen staining was quantified by digital image analysis using DP2-BSW software. Bars represent mean ± S.E. (n = 5); * p<0.001 compared with control.

Singhal J., Chikara S., Horne D., Salgia R., Awasthi S, & Singhal S.S. (2019). RLIP inhibition suppresses breast-to-lung metastasis. Cancer letters, 447, 24-32.

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Epithelial-Mesenchymal Transition in Tissues

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Tissues from control groups (corn oil and PIS) and experimental groups (2HF, Rab, and 2HF+Rab) were fixed in buffered formalin for 12 h. Paraffin-embedded 5 μm thick tissues sections were prepared. Hematoxylin and Eosin (H&E) staining to assess hyperplasia was performed on paraffin-embedded brain, heart, kidney, liver and lung sections. Histopathologic analysis of proteins such as E cadherin and vimentin involved in epithelial-mesenchymal transition (EMT), CD31 to visualize blood vessels, Ki67 & RLIP to assess cell proliferation, and vimentin to analyze mesenchymal cells was performed in brain sections using Universal ABC detection kit (Vector). Immuno-reactivity is evident as a dark brown stain, whereas non-reactive areas display only the background color. Photomicrographs at 40x magnification were acquired using Olympus DP72 microscope. Percent staining was determined by measuring positive immuno-reactivity per unit area. The intensity of antigen staining was quantified by digital image analysis using DP2-BSW software. Bars represent mean ± S.E. (n = 5); * p<0.003 compared with control.

Singhal J., Singhal P., Horne D., Salgia R., Awasthi S, & Singhal S.S. (2018). Metastasis of Breast Tumor Cells to Brain is suppressed by Targeting RLIP alone and in combination with 2’-Hydroxyflavanone. Cancer letters, 438, 144-153.

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Histopathological Analysis of Lung Tumors

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Lung tumors (control as well as 2HF treated) were harvested from mice bearing tumors and used for histopathologic analyses. Tumor samples fixed in buffered formalin for 12 h were processed conventionally for paraffin-embedded tumor sections (5 μm thick). Hematoxylin and Eosin (H&E) staining was performed on paraffin-embedded tumor sections. Histopathologic analyses with anti-E cadherin, anti-CD31, and anti-Ki67 IgG, were also performed, using Universal ABC detection kit (Vector). Immuno-reactivity is evident as a dark brown stain, whereas non-reactive areas display only the background color. Sections were counterstained with Hematoxylin (blue). Photomicrographs at 40x magnification were acquired using Olympus DP 72 microscope.

Awasthi S., Singhal S.S., Singhal J., Nagaprashantha L., Li H., Yuan Y.C., Liu Z., Berz D., Igid H., Green W.C., Tijani L., Tonk V., Rajan A., Awasthi Y, & Singh S.P. (2018). Anticancer activity of 2’-hydroxyflavanone towards lung cancer. Oncotarget, 9(90), 36202-36219.

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