All spiny mice in this study were derived from breeders obtained from the Seifert Lab at the University of Kentucky and maintained at Emory University. Sex was defined based on gonads, with males having testes present and females having ovaries present. Animals were housed in groups of 2–5 same-sex siblings in either a GR1800 Double Decker cages (Tecniplast, West Chester, PA, USA) or a standard polycarbonate rat cage. They were provided ad libitum water and Purina Prolab RMH 1000 (Lab Diet, St. Louis, MO, USA). The colony room was maintained at an ambient temperature of 24 °C ± 1 °C and kept on a 14 h:10 h light/dark cycle (lights on at 7:00 AM). All procedures were approved by the Institutional Animal Care and Use Committee of Emory University (Protocol 201,900,126).
Gr1800 double decker cage
Manufactured by Tecniplast
Sourced in United Kingdom
The GR1800 Double-Decker Cage is a rodent housing system designed by Tecniplast. It features two interconnected levels, allowing for increased cage capacity while maintaining a compact footprint. The system is constructed with durable materials and includes key components necessary for the housing and care of laboratory animals.
Automatically generated - may contain errors
Lab products found in correlation
6 protocols using gr1800 double decker cage
1
Spiny Mouse Breeding and Housing
2
Rodent Housing and Behavioral Protocols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Eight male Lister Hooded rats were used (Envigo), weighing ∼300–350 g at the time of surgery and behaviorally naive. Prior to surgery, rats were group housed. Following surgery, implanted rats were housed singly (GR1800 double-decker cages; Tecniplast) except for ∼1 h daily, supervised socialization. Animals were maintained under diurnal light conditions (14/10 h light/dark) with ad libitum access to water and environmental enrichment. When required, animals were food-deprived to no less than 85% of their ad libitum feeding weight to promote reward-based exploration.
3
Wistar Rat Gestational Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Virgin female Wistar rats (Charles River, U.K.) weighing between 220 and 315 g (mean 262.8 ± 2.8 g, N=63) were acclimatised to the housing conditions for at least one week before mating, maintained at a temperature of 21–23°C, a humidity of 55–60% with a 12 h:12 h light:dark cycle (lights on at 07:00). Female rats were mated with adult male Wistar rats (Charles River, U.K.) and pair-housed in individually ventilated cages with split-level environmental enrichment (GR1800 Double-Decker Cage, Tecniplast, U.K.), with ad libitum access to standard rat chow (Special Diet Services, U.K.) and water. Gestational day 1 (GD1) was determined by the presence of a vaginal plug.
4
Maternal Immunoactivation in Wistar Rats
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Animals used in this study were generated at the University of Manchester. Adult female Wistar rats (Charles River Laboratories, UK) were used for MIA during pregnancy. In pregnancy, rats were housed in pairs or threes before being singly housed from GD19. Rats were housed in individually ventilated cages (IVCs) with two levels (GR1800 Double-Decker Cage, Tecniplast, UK) under a standard 12- h light:dark cycle (lights on 7:00 am). The environment was maintained at 21°C ± 2°C, 55%±5% humidity. Animals had ad lib access to standard rat chow (Special Diet Services, UK) and water (Murray et al., 2019 ). All procedures in this study were carried out in accordance with the UK Home Office, Animals (Scientific Procedures) Act 1986 and EU Directive 2010/63/EU. The University of Manchester Animal Welfare and Ethical Review Body (AWERB) approved all experimental protocols used in this study.
5
Maternal Immune Activation in Wistar Rats
6
Lister Hooded Rat Housing Conditions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
These studies were performed by b-neuro at the University of Manchester. Female Lister Hooded (LH) rats (190–220 g) (n = 50 per experiment, 10 animals per group; Charles River Laboratories, UK), were housed in groups of five in individually ventilated, two-tiered plastic cages (38 cm × 59 cm × 24 cm, GR1800 Double-Decker Cage, Tecniplast, UK). These cages contained paper sizzle nest, sawdust, and cardboard tunnels (Datesand group, UK). The rats had access to water and standard rat chow (Special Diet Services) ad libitum within the home cage. The environment was kept constant at 20 ± 2 °C, 55 ± 5% humidity, under a 12:12 h light/dark cycle (lights on at 07:00).
All procedures used in these experiments were conducted in accordance with the Animals Scientific Procedures Act (London, UK, 1986) and were approved by the University of Manchester AWERB (Animal Welfare and Ethical Review Body).
No pre-established inclusion/exclusion criteria were used for the subsequent molecular analyses. All samples were processed and analysed by investigators blind to the housing conditions. All procedures were conducted in the morning (at 11:00 a.m. ± 2).
All procedures used in these experiments were conducted in accordance with the Animals Scientific Procedures Act (London, UK, 1986) and were approved by the University of Manchester AWERB (Animal Welfare and Ethical Review Body).
No pre-established inclusion/exclusion criteria were used for the subsequent molecular analyses. All samples were processed and analysed by investigators blind to the housing conditions. All procedures were conducted in the morning (at 11:00 a.m. ± 2).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!