Cells and tissue sections were fixed in 2% paraformaldehyde at room temperature for 5 minutes and immunofluorescent staining was performed as previously described.(23 (link), 24 (link)) Briefly, Samples were labeled using mouse anti-Cx43 (Millipore MAB3067, 1:100), rabbit anti-Nav1.5 (kindly provided by Dr. Peter Mohler, 1:100) and rabbit anti-Kir2.1 (Alomone Labs, APC-026, 1:100) antibodies. Goat anti-mouse AlexaFluor 546 (1:4000) and goat anti-rabbit AlexaFluor 488 (1:4000) secondary antibodies were used for confocal microscopy while goat anti-rabbit Alexa 647 (1:4000) and donkey anti-mouse Cy3b (1:100) secondary antibodies were used for super-resolution ST ochastic O ptical R econstruction M icroscopy (STORM). Goat anti-rabbit Chromeo 505 (1:100) and anti-mouse biotin (1:200) followed by streptavidin-conjugated Horizon V500 (1:100) secondary antibodies were used for g ated ST imulated E mission D epletion (gSTED) microscopy.
Apc 026
Manufactured by Alomone
Sourced in Israel
APC-026 is a fluorochrome-conjugated antibody that binds to the apoptosis-associated protein APC. It is a tool for detecting and measuring apoptosis in flow cytometry applications.
Automatically generated - may contain errors
3 protocols using apc 026
1
Multi-Protein Immunofluorescence Microscopy
2
Western Blot Analysis of Ion Channels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Western blot analysis was performed on total proteins extracted from AC16 cells. Sixty milligrams of protein was equally loaded on 10% SDS-polyacrylamide gel and then transferred onto a nitrocellulose filter membrane. After getting the target bands, the membranes were blocked by 5% filtered nonfat milk for 2 h and then incubated with the indicated primary antibodies overnight at 4°C, including rabbit anti-NaV1.5 antibody (ASC-005, Alomone Labs, Israel, 1: 200), rabbit anti-Kir2.1 antibody (APC-026, Alomone Labs, Israel, 1: 200) followed by incubation with the corresponding secondary antibody for 1 h at room temperature. Protein levels were quantified using the Odyssey Infrared Imaging System (LI-COR, 4607 Superior Street, P.O. Box 4,000, Lincoln, Nebraska, United States) by measuring proteins’ gray values and the data was normalized to β-actin (TA-09, Zhongshanjinqiao, Beijing, China, 1:1,000) as an internal control.
3
Immunostaining of Cardiac Ion Channels
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
AC16 cells were fixed in 4% paraformaldehyde (PFA) and permeabilized with 0.4% Triton-X at room temperature for 20 min. The samples were coincubated with NaV1.5 (ASC-005, Alomone Labs, Israel, 1:200), Kir2.1 (APC-026, Alomone Labs, Israel, 1: 200), and α-actinin (ab50599, Abcam, Cambridge, United Kingdom, 1:100) antibody at 4°C overnight. Then the AC16 cells were incubated with the appropriate secondary antibody at room temperature for 2 h and the nuclei were stained with DAPI (Beyotime, Shanghai, China, 1:1,000) for 10 min. Immunofluorescence was observed under a confocal laser scanning microscope (Nikon 80i, Otawara, Tochigi, Japan).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!