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Ae31e inverted microscope

Manufactured by Motic
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Sourced in Spain

The AE31E is an inverted microscope from Motic. It is designed for observation and documentation of samples. The microscope features an LED illumination system and a trinocular observation head.

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Inverted microscope (25 citations) AE31E (15 citations) AE31E microscope (5 citations) AE31E Trinocular Inverted Microscope (2 citations)

2 protocols using ae31e inverted microscope

1

Evaluating Cell Viability with Fluorescent Dyes

Cited 1 time
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To evaluate cell viability, the cells were washed three times with Hank’s balanced salt solution and incubated with 5 µM propidium iodide (λex = 493 nm, λem 636 nm) and 5 µg/mL of Hoechst 33342 vital dye (λex = 361 nm, λem 497 nm) at 37 °C. Fluorescence levels were detected using an LED imaging system based on an AE31E inverted microscope (Motic, Barselona, Spain) [28 (link)]. Analysis of the images obtained was performed using the Image J2 (Fiji) software version 2.3.1 (NIH, Bethesda, MD, USA). In each sample 200–400 cells were analyzed.
Belosludtsev K.N., Serov D.A., Ilzorkina A.I., Starinets V.S., Dubinin M.V., Talanov E.Y., Karagyaur M.N., Primak A.L, & Belosludtseva N.V. (2023). Pharmacological and Genetic Suppression of VDAC1 Alleviates the Development of Mitochondrial Dysfunction in Endothelial and Fibroblast Cell Cultures upon Hyperglycemic Conditions. Antioxidants, 12(7), 1459.
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2

Integrin β1 Dependent Laminin Adhesion

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HDMECs were harvested, incubated with anti-integrin β1 (clone P4C10, NBP2-36561, Biotechne) or isotype control mouse IgG1 (P3.6.2.8.1; 14-4714-81 from Invitrogen) for 5 minutes, then 1.5 × 104 cells were added to the wells of a 96 well plate that had been coated or not with different laminins as described above. After 1 hour, each well was washed three times with serum free medium and attached cells were imaged with a digital microscope camera (Micropix) attached to an AE31E inverted microscope (Motic). The cell count per image was determined using ImageJ.
Hsieh L.T., Hall B.S., Newcombe J., Mendum T.A., Umrania Y., Deery M.J., Shi W.Q., Salguero F.J, & Simmonds R.E. (2023). Mycolactone causes catastrophic Sec61-dependent loss of the endothelial glycocalyx and basement membrane: a new indirect mechanism driving tissue necrosis in Mycobacterium ulcerans infection. bioRxiv.
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