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Ab37068

Manufactured by Abcam
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Ab37068 is an immunohistochemistry primary antibody that detects the presence of a specific protein target in tissue samples. It can be used to identify the localization and expression levels of the target protein within the sample.

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Lab products found in correlation

β actin antibody, by Cell Signaling Technology (1 mentions) Anti histone h3, by Cell Signaling Technology (1 mentions) Anti phospho nf κb p65, by Cell Signaling Technology (1 mentions) Ab263882, by Abcam (1 mentions) Ab34139, by Abcam (1 mentions) Ab100747, by Abcam (1 mentions) Ab234579, by Abcam (1 mentions) Ab100712, by Abcam (1 mentions) Ab33471, by Abcam (1 mentions) Ab223588, by Abcam (1 mentions) Ab100705, by Abcam (1 mentions) Ab199081, by Abcam (1 mentions)

2 protocols using ab37068

1

Protein Extraction and Western Blot Analysis

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BMDCs protein was extracted using the Nuclear and Cytoplasmic Protein Extraction Kit (Cat. P0027) and the RIPA Lysis Buffer (Cat. P0013C) from Beyotime Institute of Biotechnology (China). Proteins were confirmed by WB analysis using an anti-TLR1 antibody (Abcam, ab37068), anti-TLR2 antibody (Abcam, ab209216), anti-TLR6 antibody (Cell Signaling Technology #12717), β-actin antibody (Cell Signaling Technology #4967), anti-Phospho-NF-κB p65 (Cell Signaling Technology #3033S) and anti-Histone H3 (Cell Signaling Technology #4499S). All antibodies were diluted 1:1000.
Zhu J., Ke Y., Liu Q., Yang J., Liu F., Xu R., Zhou H., Chen A., Xiao J., Meng F., Yu L., Li R., Wei J, & Liu B. (2022). Engineered Lactococcus lactis secreting Flt3L and OX40 ligand for in situ vaccination-based cancer immunotherapy. Nature Communications, 13, 7466.
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2

Serum Biomarker Profiling in Rats

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On week 4, blood samples (1 ml) were collected through cardiac puncture during sacrifice and serum was separated using centrifugation at 10,000 × g for 5 min at 4°C. Serum levels of TNF-α (ab100747, Abcam), IL-1β (ab100705, Abcam), IL-17A (ab199081, Abcam), IL-6 (ab100712, Abcam), TGF-β1 (ab118557, Abcam), IL-2 (ab223588, Abcam), IL-10 (ab33471, Abcam), ALT (ab234579, Abcam), AST (ab263882, Abcam), γ-GT (ab134640, Abcam), ALP (ab256583, Abcam), DBIL (ab34139, Abcam) and TBIL (ab37068, Abcam) were measured by using rat-specific sandwich ELISA (Abcam) according to the manufacturer's protocol.
Zhou Y., Lian H., Liu K., Wang D., Xiu X, & Sun Z. (2020). Puerarin improves graft bone defect through microRNA-155-3p-mediated p53/TNF-α/STAT1 signaling pathway. International Journal of Molecular Medicine, 46(1), 239-251.
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